5540767
HSP27 Antibody (HSPB1/774), Novus Biologicals™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
HSP27
Concentration
0.2mg/mL
Applications
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), SDS-Page, Immunofluorescence
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Cancer, Cell Biology, Cell Cycle and Replication, Golgi Apparatus Markers, Membrane Trafficking and Chaperones, Neuroscience
Formulation
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias
28 kDa heat shock protein, DKFZp586P1322, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, heat shock 27kD protein 1, heat shock 27kDa protein 1, heat shock protein beta-1, HMN2B, HS.76067, Hsp25, HSP27HSP 27, HSP28CMT2F, HspB1, SRP27, Stress-responsive protein 27
Gene Symbols
HSPB1
Isotype
IgG1 κ
Purification Method
Protein A or G purified
Test Specificity
It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced p29 and 24K protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
Clone
HSPB1/774
Dilution
Western Blot 0.25 - 0.5 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human, Mouse, Rat, Chicken, Primate, Sheep
Gene Accession No.
P04792
Gene ID (Entrez)
3315
Immunogen
Recombinant human HSPB1 protein
Primary or Secondary
Primary
Content And Storage
Store at 4C.
Molecular Weight of Antigen
27 kDa
Related Products
Description
- Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence HSP27 Monoclonal specifically detects HSP27 in Human, Mouse, Rat, Chicken, Chimpanzee, Sheep samples
- It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence.
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Antigen: HSP27
Concentration: 0.2mg/mL
Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), SDS-Page, Immunofluorescence
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cancer, Cell Biology, Cell Cycle and Replication, Golgi Apparatus Markers, Membrane Trafficking and Chaperones, Neuroscience
Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias: 28 kDa heat shock protein, DKFZp586P1322, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, heat shock 27kD protein 1, heat shock 27kDa protein 1, heat shock protein beta-1, HMN2B, HS.76067, Hsp25, HSP27HSP 27, HSP28CMT2F, HspB1, SRP27, Stress-responsive protein 27
Gene Symbols: HSPB1
Isotype: IgG1 κ
Purification Method: Protein A or G purified
Test Specificity: It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced p29 and 24K protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
Clone: HSPB1/774
Dilution: Western Blot 0.25 - 0.5 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Mouse, Rat, Chicken, Primate, Sheep
Gene Accession No.: P04792
Gene ID (Entrez): 3315
Immunogen: Recombinant human HSPB1 protein
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Molecular Weight of Antigen: 27 kDa
Antigen:
HSP27
Concentration:
0.2mg/mL
Applications:
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), SDS-Page, Immunofluorescence
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Cancer, Cell Biology, Cell Cycle and Replication, Golgi Apparatus Markers, Membrane Trafficking and Chaperones, Neuroscience
Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias:
28 kDa heat shock protein, DKFZp586P1322, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, heat shock 27kD protein 1, heat shock 27kDa protein 1, heat shock protein beta-1, HMN2B, HS.76067, Hsp25, HSP27HSP 27, HSP28CMT2F, HspB1, SRP27, Stress-responsive protein 27
Gene Symbols:
HSPB1
Isotype:
IgG1 κ
Purification Method:
Protein A or G purified
Test Specificity:
It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced p29 and 24K protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
Clone:
HSPB1/774
Dilution:
Western Blot 0.25 - 0.5 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Mouse, Rat, Chicken, Primate, Sheep
Gene Accession No.:
P04792
Gene ID (Entrez):
3315
Immunogen:
Recombinant human HSPB1 protein
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Molecular Weight of Antigen:
27 kDa
Antigen: HSP60
Concentration: 0.2 mg/mL
Applications: Flow Cytometry, Immunofluorescence
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Apoptosis, Cellular Markers, Membrane Trafficking and Chaperones, Mitochondrial Markers
Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias: 60 kDa chaperonin, Chaperonin 60, CPN60, GROEL, heat shock 60kD protein 1 (chaperonin), heat shock 60kDa protein 1 (chaperonin), Heat shock protein 60, heat shock protein 65, HLD4, Hsp60, HSP-60, HSP60SPG13, HSP65, HuCHA60, Mitochondrial matrix protein P1,60 kDa heat shock protein, mitochondrial, P60 lymphocyte protein, short heat shock protein 60 Hsp60s1, spastic paraplegia 13 (autosomal dominant)
Gene Symbols: HSPD1
Isotype: IgG1 κ
Purification Method: Protein A or G purified
Test Specificity: Recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsps), more appropriately called as stress response proteins (srps). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog.
Clone: HSPD1/875
Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence 0.5 - 1.0 ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Mouse, Rat, Chicken, Guinea Pig, Hamster, Monkey
Gene Accession No.: P10809
Gene ID (Entrez): 3329
Immunogen: Recombinant human HSPD1 protein
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Molecular Weight of Antigen: 60 kDa
Antigen:
HSP60
Concentration:
0.2 mg/mL
Applications:
Flow Cytometry, Immunofluorescence
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Apoptosis, Cellular Markers, Membrane Trafficking and Chaperones, Mitochondrial Markers
Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias:
60 kDa chaperonin, Chaperonin 60, CPN60, GROEL, heat shock 60kD protein 1 (chaperonin), heat shock 60kDa protein 1 (chaperonin), Heat shock protein 60, heat shock protein 65, HLD4, Hsp60, HSP-60, HSP60SPG13, HSP65, HuCHA60, Mitochondrial matrix protein P1,60 kDa heat shock protein, mitochondrial, P60 lymphocyte protein, short heat shock protein 60 Hsp60s1, spastic paraplegia 13 (autosomal dominant)
Gene Symbols:
HSPD1
Isotype:
IgG1 κ
Purification Method:
Protein A or G purified
Test Specificity:
Recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsps), more appropriately called as stress response proteins (srps). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog.
Clone:
HSPD1/875
Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence 0.5 - 1.0 ug/ml
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Mouse, Rat, Chicken, Guinea Pig, Hamster, Monkey
Gene Accession No.:
P10809
Gene ID (Entrez):
3329
Immunogen:
Recombinant human HSPD1 protein
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Molecular Weight of Antigen:
60 kDa
Antigen: HSP60
Concentration: 0.2 mg/mL
Applications: Flow Cytometry, Immunofluorescence
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Apoptosis, Cellular Markers, Membrane Trafficking and Chaperones, Mitochondrial Markers
Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias: 60 kDa chaperonin, Chaperonin 60, CPN60, GROEL, heat shock 60kD protein 1 (chaperonin), heat shock 60kDa protein 1 (chaperonin), Heat shock protein 60, heat shock protein 65, HLD4, Hsp60, HSP-60, HSP60SPG13, HSP65, HuCHA60, Mitochondrial matrix protein P1,60 kDa heat shock protein, mitochondrial, P60 lymphocyte protein, short heat shock protein 60 Hsp60s1, spastic paraplegia 13 (autosomal dominant)
Gene Symbols: HSPD1
Isotype: IgG1 κ
Purification Method: Protein A or G purified
Test Specificity: Recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsps), more appropriately called as stress response proteins (srps). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog.
Clone: HSPD1/875
Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence 0.5 - 1.0 ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Mouse, Rat, Chicken, Guinea Pig, Hamster, Monkey
Gene Accession No.: P10809
Gene ID (Entrez): 3329
Immunogen: Recombinant human HSPD1 protein
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Molecular Weight of Antigen: 60 kDa
Antigen:
HSP60
Concentration:
0.2 mg/mL
Applications:
Flow Cytometry, Immunofluorescence
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Apoptosis, Cellular Markers, Membrane Trafficking and Chaperones, Mitochondrial Markers
Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias:
60 kDa chaperonin, Chaperonin 60, CPN60, GROEL, heat shock 60kD protein 1 (chaperonin), heat shock 60kDa protein 1 (chaperonin), Heat shock protein 60, heat shock protein 65, HLD4, Hsp60, HSP-60, HSP60SPG13, HSP65, HuCHA60, Mitochondrial matrix protein P1,60 kDa heat shock protein, mitochondrial, P60 lymphocyte protein, short heat shock protein 60 Hsp60s1, spastic paraplegia 13 (autosomal dominant)
Gene Symbols:
HSPD1
Isotype:
IgG1 κ
Purification Method:
Protein A or G purified
Test Specificity:
Recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsps), more appropriately called as stress response proteins (srps). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog.
Clone:
HSPD1/875
Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence 0.5 - 1.0 ug/ml
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Mouse, Rat, Chicken, Guinea Pig, Hamster, Monkey
Gene Accession No.:
P10809
Gene ID (Entrez):
3329
Immunogen:
Recombinant human HSPD1 protein
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Molecular Weight of Antigen:
60 kDa
Antigen: Macrophage and Histiocytoma Marker
Concentration: 0.2mg/mL
Applications: Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: __
Formulation: 10mM PBS with 0.05% Sodium Azide
Gene Alias: __
Gene Symbols: __
Isotype: IgG1 κ
Purification Method: Protein A or G purified
Test Specificity: In Western blotting, it detects an antigen of 125kDa in human liver and 135kDa in tumors of histiocytic origin. Comparative study of this MAb and a standard CD68 MAb showed that their antigens are different. Its antigen in all macrophage types studied is located on the plasma membrane and within cytoplasmic structures including lysosomes. This MAb shows a restricted reactivity to cells of the monocyte/macrophage system. It specifically reacts with blood monocytes and stains resident macrophages in a wide variety of human tissues. This MAb does not stain antigen-presenting cells, e.g., Langerhans cells. Reportedly, its reactivity is restricted to histiocytes and macrophages.
Clone: D11
Dilution: Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Mouse (Negative), Porcine (Negative), Rat (Negative)
Gene Accession No.: __
Gene ID (Entrez): __
Immunogen: Membrane preparation from human hepatocytes
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Molecular Weight of Antigen: __
Antigen:
Macrophage and Histiocytoma Marker
Concentration:
0.2mg/mL
Applications:
Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
__
Formulation:
10mM PBS with 0.05% Sodium Azide
Gene Alias:
__
Gene Symbols:
__
Isotype:
IgG1 κ
Purification Method:
Protein A or G purified
Test Specificity:
In Western blotting, it detects an antigen of 125kDa in human liver and 135kDa in tumors of histiocytic origin. Comparative study of this MAb and a standard CD68 MAb showed that their antigens are different. Its antigen in all macrophage types studied is located on the plasma membrane and within cytoplasmic structures including lysosomes. This MAb shows a restricted reactivity to cells of the monocyte/macrophage system. It specifically reacts with blood monocytes and stains resident macrophages in a wide variety of human tissues. This MAb does not stain antigen-presenting cells, e.g., Langerhans cells. Reportedly, its reactivity is restricted to histiocytes and macrophages.
Clone:
D11
Dilution:
Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Mouse (Negative), Porcine (Negative), Rat (Negative)
Gene Accession No.:
__
Gene ID (Entrez):
__
Immunogen:
Membrane preparation from human hepatocytes
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Molecular Weight of Antigen:
__