5540643

Golgi Complex Antibody (AE-6), Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

Golgi Complex

Concentration

0.2mg/mL

Applications

Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, Immunocytochemistry

Conjugate

Unconjugated

Host Species

Mouse

Target Species

Human, Mouse (Negative), Rat (Negative)

Immunogen

SU-DHL-1 large cell lymphoma cells

Primary or Secondary

Primary

Content And Storage

Store at 4C.

Clone

AE-6

Dilution

Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml, Immunocytochemistry 0.5 - 1.0 ug/ml

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Formulation

10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Isotype

IgG1 κ

Purification Method

Protein A or G purified

Test Specificity

This MAb recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside

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Description

  • Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunocytochemistry, Immunofluorescence Golgi Complex Monoclonal specifically detects Golgi Complex in Human, Mouse (Negative), Rat (Negative) samples
  • It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence, Immunocytochemistry.

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Fischer Scientific

5540643

Mouse Monoclonal Antibody...


Antigen:
Golgi Complex

Concentration:
0.2mg/mL

Applications:
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, Immunocytochemistry

Conjugate:
Unconjugated

Host Species:
Mouse

Target Species:
Human, Mouse (Negative), Rat (Negative)

Immunogen:
SU-DHL-1 large cell lymphoma cells

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Clone:
AE-6

Dilution:
Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml, Immunocytochemistry 0.5 - 1.0 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Isotype:
IgG1 κ

Purification Method:
Protein A or G purified

Test Specificity:
This MAb recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside

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Antigen:
Golgi Glycoprotein 1/GLG1

Concentration:
0.2mg/mL

Applications:
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, Immunocytochemistry

Conjugate:
Unconjugated

Host Species:
Mouse

Target Species:
Human, Mouse (Negative), Rat (Negative)

Immunogen:
Golgi fraction from human liver cells

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Clone:
GLG1/970

Dilution:
Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml, Immunocytochemistry 0.5 - 1.0 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Isotype:
IgG1 κ

Purification Method:
Protein A or G purified

Test Specificity:
This MAb recognizes a protein of 134kDa, which binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils). Fucosylation is essential for binding to E-selectin. It contains sialic acid residues and 16 Cys-rich GLG1 repeats. This MAb can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three fun

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Antigen:
__

Concentration:
__

Applications:
__

Conjugate:
__

Host Species:
__

Target Species:
__

Immunogen:
__

Primary or Secondary:
__

Content And Storage:
__

Clone:
__

Dilution:
__

Classification:
__

Form:
__

Regulatory Status:
__

Formulation:
__

Isotype:
__

Purification Method:
__

Test Specificity:
__

Img

Fischer Scientific

5540657

Rat Monoclonal Antibody...


Antigen:
gp96/HSP90B1/GRP94

Concentration:
0.2mg/mL

Applications:
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Rat

Target Species:
Human, Mouse, Rat, Porcine, Canine, Chicken, Equine, Guinea Pig, Hamster, Monkey, Primate, Rabbit, Sheep, Xenopus species

Immunogen:
Purified glucose regulated protein 94 (grp94) from chicken oviducts

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Clone:
9G10.F8.2

Dilution:
Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Isotype:
IgG2a κ

Purification Method:
Protein A or G purified

Test Specificity:
Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This MAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grp s are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.