Nucleolin Antibody (NCL/902), Novus Biologicals™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
Nucleolin
Concentration
0.2mg/mL
Applications
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Cell Cycle and Replication, Chromatin Research, Core ESC Like Genes, DNA Repair, DNA replication Transcription Translation and Splicing, Stem Cell Markers
Formulation
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias
C23, FLJ45706, FLJ59041, nucleolin, Protein C23
Gene Symbols
NCL
Isotype
IgG1 κ
Purification Method
Protein A or G purified
Test Specificity
Recognizes a protein of ∼76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Clone
NCL/902
Dilution
Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human, Bovine (Negative), Mouse (Negative), Rat (Negative)
Gene Accession No.
P19338
Gene ID (Entrez)
4691
Immunogen
Recombinant human NCL protein
Primary or Secondary
Primary
Content And Storage
Store at 4C.
Molecular Weight of Antigen
76 kDa
Related Products
Description
- Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence Nucleolin Monoclonal specifically detects Nucleolin in Human, Bovine (Negative), Mouse (Negative), Rat (Negative) samples
- It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.
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Antigen: Nucleolin
Concentration: 0.2mg/mL
Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cell Cycle and Replication, Chromatin Research, Core ESC Like Genes, DNA Repair, DNA replication Transcription Translation and Splicing, Stem Cell Markers
Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias: C23, FLJ45706, FLJ59041, nucleolin, Protein C23
Gene Symbols: NCL
Isotype: IgG1 κ
Purification Method: Protein A or G purified
Test Specificity: Recognizes a protein of ∼76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Clone: NCL/902
Dilution: Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Bovine (Negative), Mouse (Negative), Rat (Negative)
Gene Accession No.: P19338
Gene ID (Entrez): 4691
Immunogen: Recombinant human NCL protein
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Molecular Weight of Antigen: 76 kDa
Antigen: NuMA
Concentration: 0.2 mg/mL
Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Breast Cancer, Cell Biology, Cell Cycle and Replication, Cellular Markers, Mitotic Regulators
Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias: centrophilin stabilizes mitotic spindle in mitotic cells, nuclear mitotic apparatus protein 1, NUMA, NuMA protein, SP-H antigen, structural nuclear protein
Gene Symbols: NUMA1
Isotype: IgM κ
Purification Method: Protein A or G purified
Test Specificity: Recognizes a phosphorylated protein of 228kDa, identified as nuclear mitotic apparatus protein (NuMA). Its epitope is resistant to phosphatases. NuMA is intra-nuclear protein and present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. After anaphase, the protein redistributes from the spindle polar region into reforming nucleus. NuMA is an essential protein during mitosis for the terminal phases of chromosome separation and/or nuclear reassembly. Recently a study shows that NuMA is cleaved to a 180 to 200kDa during apoptosis. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 has been detected in patients with acute promyelocytic leukemia.
Clone: SPM300
Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Accession No.: __
Gene ID (Entrez): 4926
Immunogen: Colon carcinoma 174T cells
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Molecular Weight of Antigen: 228 kDa
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Immunogen: __
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