CD71 (Transferrin Receptor) Monoclonal Antibody (R17217 (RI7 217.1.4)), Alexa Fluor™ 700, eBioscience™, Invitrogen™

Description

• Description: The R17217 monoclonal antibody reacts with mouse CD71, a 170-180 kDa type II transmembrane protein
• CD71, the transferrin receptor, exists as a homodimer on the cell surface and is essential for cellular growth
• CD71 is expressed by immature proliferating cells and at low levels on resting mature lymphocytes
• Antigen or mitogen stimulation of T and B cells upregulates the expression of CD71
• Expression level differences have been observed in different mouse strains
• Applications Reported: This R17217 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This R17217 antibody has been tested by flow cytometric analysis of mouse bone marrow cells
• This may be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Alexa Fluor 700 emits at 723 nm and can be excited with the red laser (633-647 nm)
• Most instruments will require a 685 LP mirror and 710/20 band pass filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes
• Endosomal acidification leads to iron release
• The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor
• Transferrin receptor is necessary for development of erythrocytes and the nervous system
• Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.