CD101 Monoclonal Antibody (Moushi101), Alexa Fluor™ 700, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody Moushi101 recognizes mouse CD101, also known as IGSF2 and V7 (7 IgV domains)
• CD101 is a transmembrane glycoprotein containing 7 Ig-like IgV domains and a short cytoplasmic tail with several phosphorylation sites
• Cross-linking CD101 can antagonize TCR/CD3-mediated lymphocyte activation
• CD101 is expressed on monocytes, granulocytes, dendritic cells, Langerhans cells and activated T cells
• In addition, CD101 is found on a subset of CD25+Foxp3+ T regulatory cells that showed higher suppressive properties in vitro and in vivo
• Applications Reported: This Moushi101 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This Moushi101 antibody has been tested by flow cytometric analysis of mouse bone marrow cells
• This may be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Alexa Fluor 700 emits at 723 nm and can be excited with the red laser (633-647 nm)
• Most instruments will require a 685 LP mirror and 710/20 band pass filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser CD101 also known as V7 antigen, a member of the Ig superfamily
• CD101 is a disulfide linked homodimer of unknown function
• Expression of CD101 is found on monocytes, granulocytes and dendritic cells (Langerhan-like cells HLA-DR, CD1a, CD1c)
• In addition expression on T lymphocytes is important for cell activation
• CD101+ CD28+ cells are very responsive to CD28 signaling
• In combination with anti-CD28 or suboptimal levels of anti-CD3, anti-CD101 can increase proliferation thereby suggesting an activating role
• The monoclonal antibody BB27 has been shown to inhibit the T cell reactivity in allogeneic and antigen-specific mixed DC-T cell cultures
• Recently it has been demonstrated that mouse CD101 is found on a subpopulation of regulatory T cells (CD4, CD25, Foxp3 positive) that have high suppressor activity
• Expression of CD101 on human PBMCs shows staining of about 30% of the Foxp3 positive cells
• Studies have not confirmed higher suppressor activity in the human CD101 population.