RELM alpha Monoclonal Antibody (DS8RELM), Alexa Fluor™ 700, eBioscience™, Invitrogen™

Description

• Description: This DS8RELM monoclonal antibody recognizes mouse RELM alpha, also known as FIZZ1
• This antibody was shown not to cross-react with mouse RELM gamma
• Applications Reported: This DS8RELM antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This DS8RELM antibody has been tested by intracellular staining followed by flow cytometric analysis of resident peritoneal macrophages using the Intracellular Fixation & Permeabilization Buffer Set (Product No
• 88-8824-00) and protocol
• Please refer to Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins located at www.thermofisher.com/flowprotocols
• This may be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Alexa Fluor 700 emits at 723 nm and can be excited with the red laser (633-647 nm)
• Most instruments will require a 685 LP mirror and 710/20 band pass filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• RELM alpha, also known as Resistin-like alpha or FIZZ1, is a small cytokine (88 aminoacids, MW 9.4 kDa) produced mostly by macrophages
• Its highest constitutive expression has been observed in small peritoneal macrophages, alveolar macrophages and adipose tissue macrophages
• It is strongly induced by IL-4 and IL-13
• Together with Arginase 1 and Ym-1, RELM alpha is considered one of the hallmarks of the M2 polarization of mouse macrophages, however, macrophages polarized with IL-10 (M2c) express neither RELM alpha nor Arginase 1
• Also, the expression patterns of RELM alpha and Arginase 1 in untreated tissue macrophages significantly differ
• Elevated levels of RELM alpha may indicate parasitic infection
• Humans do not have RELM alpha gene but human Resistin resembles mouse RELM alpha in respect to sequence, tissue distribution and function
• Other members of RELM family in mouse: RELM beta, RELM gamma and Resistin display different tissue distribution and functional properties
• RELM alpha plays an important role in parasite infection, mucosal inflammation and allergy.