IgD Monoclonal Antibody (11-26c (11-26)), Alexa Fluor™ 700, eBioscience™, Invitrogen™

Description

• Description: The 11-26c monoclonal antibody reacts with the delta heavy chain of mouse IgD
• It does not react with other classes of mouse immunoglobulin including IgA, IgG or IgM
• IgD is expressed by peripheral mature B cells
• 11-26c does not activate B cells
• Applications Reported: This 11-26 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 11-26 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This may be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Alexa Fluor 700 emits at 723 nm and can be excited with the red laser (633-647 nm)
• Most instruments will require a 685 LP mirror and 710/20 band pass filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM
• IgD also exists as a soluble form in blood serum
• Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.