TER-119 Monoclonal Antibody (TER-119), eFluor™ 506, eBioscience™, Invitrogen™

Description

• Description: The TER-119 monoclonal antibody reacts with mouse erythroid cells from early proerythroblast to mature erythrocyte stages
• The TER-119 antigen is present in yolk sac, fetal and newborn liver, but is not expressed by cells carrying BFU-E and CFU-E activities
• Several erythroleukemia cell lines tested so far are negative for expression of TER-119 antigen even after dimethylsulfoxide stimulation
• Biochemical and molecular analysis of the TER-119 antigen indicate that this molecule is associated with the surface glycophorin A, but is not a typical glycophorin
• Applications Reported: This TER-119 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This TER-119 antibody has been tested by flow cytometric analysis of mouse bone marrow cells
• This may be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• eFluor™ 506 can be excited with the violet laser line (405 nm) and emits at 506 nm
• We recommend using a 510/20 band pass filter, or equivalent
• Please make sure that your instrument is capable of detecting this fluorochrome
• Excitation: 405 nm; Emission: 506 nm; Laser: Violet Laser TER-119 is a lineage marker for erythroid cells from early proerythroblast to mature erythrocyte stages in adult blood, spleen, and bone marrow
• It is also present in yolk sac, and fetal and newborn liver
• The TER-119 antigen is not expressed by cells of earlier erythroid development at BFU-e (blast-forming unit erythroid) stage or CFU-e (colony-forming unit erythroid) stage.