Ly-6G Monoclonal Antibody (1A8-Ly6g), Alexa Fluor™ 700, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody 1A8-Ly6g reacts with mouse Ly-6G, which is also known as Gr-1
• Ly-6G is a 25-kDa GPI protein expressed exclusively by neutrophils
• Studies suggest an important role in neutrophil infiltration, recruitment and migration
• Ly-6G associates with B2 integrins CD11a and CD11b and can attenuate B2 integrin expression and function
• The ligand for Ly-6G is currently unknown
• Unlike the RB6-8C5 antibody, 1A8-Ly6g is reported to only bind to Ly-6G found on neutrophils
• Moreover, 1A8-Ly6g has been used to deplete neutrophils and reported to have antagonist activity
• Applications Reported: This 1A8-Ly6G antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 1A8-Ly6G antibody has been tested by flow cytometric analysis of mouse bone marrow cells
• This may be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Alexa Fluor 700 emits at 723 nm and can be excited with the red laser (633-647 nm)
• Most instruments will require a 685 LP mirror and 710/20 band pass filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser Ly6G (Lymphocyte antigen 6 complex locus G6D) is a component of the myeloid differentiation antigen Gr-1, together with Ly6C
• The Ly6G protein has a molecular weight of approximately 21-25 kDa and is expressed by myeloid derived cells in the bone marrow
• Ly6G is a good marker for detection of peripheral neutrophils, monocytes and granulocytes
• Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation
• The physiological role of Ly6G remains unclear but studies have shown its involvement in anti-tumor response, and studies with neutrophils suggest that Ly6G ligation may have an inhibitory effect on local immune responses.