Ki-67 Monoclonal Antibody (SolA15), Alexa Fluor™ 532, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein
• Ki-67 is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0)
• Ki-67 is detected within the nucleus during interphase but redistributes to the chromosomes during mitosis
• Ki-67 is used as a marker for determining the growth fraction of a given population of cells
• In studies of tumor cells, the Ki-67 labeling index refers to the number of Ki-67 positive cells within the population and this is used to predict outcome of particular cancer types
• Ki-67 has been shown to interact with the DNA-bound protein chromobox protein homolog 3 (CBX3) (heterochromatin)
• The SolA15 antibody also recognizes human, non-human primate and canine Ki-67
• Applications Reported: This SOLA15 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This SOLA15 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes
• This may be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Alexa Fluor 532 is excited with the Green laser (532 nm) and emits at 561 nm
• Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases
• The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus
• Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase
• Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis
• Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms
• In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index
• The correlation between low Ki-67 index and histologically low-grade tumors is strong
• Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.