CD24 Monoclonal Antibody (M1/69), PE-eFluor™ 610, eBioscience™, Invitrogen™

Description

• Description: The M1/69 monoclonal antibody reacts with the mouse CD24 molecule, also known as Heat Stable Antigen (HSA)
• This 35-50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes and myeloid lineage
• CD24 is a variably glycosylated molecule resulting in heterogeneity of molecular mass of this antigen on cells of different lineages and antibodies to CD24 exhibit subtle differences in staining level on lymphocyte populations
• The expression of CD24 has been used to resolve stages of B lymphopoiesis in mouse bone marrow
• It has been reported that P-selectin (CD62P) binds to CD24
• Applications Reported: This M1/69 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This M1/69 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This may be used at less than or equal to 0.06 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• PE-eFluor 610 can be excited with laser lines from 488-561 nm and emits at 607 nm
• We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red)
• CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, beginning with the bone marrow pro-B-cell compartment and continuing through mature, surface Ig positive B-cells
• Plasma cell expression is very low or negative
• CD24 is also expressed on the majority of B-lineage acute lymphoblastic leukemias, B-cell CCLs and B-cell non-Hodgkin's lymphomas
• CD24 may play a role in regulation of B-cell proliferation and maturation, and control of autoimmunity.