CD25 Monoclonal Antibody (CD25-4E3), PE-eFluor™ 610, eBioscience™, Invitrogen™

Description

• Description: This CD25-4E3 monoclonal antibody reacts to human CD25, which is also known as the low-affinity interleukin (IL)-2 receptor alpha
• CD25 is expressed on activated T cells (including a subset of regulatory T cells), B cells, and macrophages
• This receptor subunit associates with CD122, the IL-2 receptor beta chain, and CD132, the common gamma chain, to form the high-affinity IL-2 receptor
• IL-2 receptor signaling has been linked to cell proliferation and survival in lymphocytes
• CD25 is a marker for Hodgkin's disease, and has been associated with such autoimmune diseases as Type I diabetes
• This monoclonal antibody is cross-reactive to rhesus monkey
• Applications Reported: This CD25-4E3 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This CD25-4E3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cels
• This can be used at 5 μL (0.125 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• PE-eFluor™ 610 can be excited with laser lines from 488-561 nm and emits at 607 nm
• We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red™)
• Please make sure that your instrument is capable of detecting this fluorochome
• CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes
• The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7)
• IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor
• Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor
• The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene
• IL2 is primarily produced by mature T cells
• IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death
• IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation
• The targeted disruption of a similar gene in mice leads to μgcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli
• Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.