CD25 Monoclonal Antibody (BC96), Super Bright™ 702, eBioscience™, Invitrogen™

Description

• Description: The LG.7F9 monoclonal antibody reacts with mouse CD27, a lymphocyte-specific member of the TNFR superfamily
• CD27 is expressed by virtually all mature T cells and by a subpopulation of B cells, mainly memory B cells
• In mouse, CD27 has been found on nearly all thymocytes excluding a population of CD46-CD8- precursors
• CD27 binds to CD70 and, through this interaction, plays an important role in T cell-B cell interaction
• It has been reported that triggering CD27 plays an important role in the maturation of CD4+ and CD8+ effector cells
• LG.7F9 cross-reacts with human and rat CD27
• Applications Reported: This LG.7F9 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This LG.7F9 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This may be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Super Bright 702 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 702 nm
• We recommend using a 710/50 bandpass filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes
• The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7)
• IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor
• Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor
• The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene
• IL2 is primarily produced by mature T cells
• IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death
• IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation
• The targeted disruption of a similar gene in mice leads to μgcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli
• Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.