62-598-182

Ly-6A/E (Sca-1) Monoclonal Antibody (D7), Super Bright™ 436, eBioscience™, Invitrogen™

Manufacturer: Invitrogen

Select a Size

Pack Size SKU Availability Price
Each of 1 62-598-182-Each-of-1 In Stock ₹ 31,913.88

62-598-182 - Each of 1

₹ 31,913.88

In Stock

Quantity

1

Base Price: ₹ 31,913.88

GST (18%): ₹ 5,744.498

Total Price: ₹ 37,658.378

Antigen

Ly-6A/E (Sca-1)

Classification

Monoclonal

Concentration

0.2 mg/mL

Formulation

PBS with BSA and 0.09% sodium azide; pH 7.2

Gene Accession No.

P05533, Q64253

Gene Symbols

Ly6a, Ly6e

Purification Method

Affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

110454, 17069

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Form

Liquid

Applications

Flow Cytometry

Clone

D7

Conjugate

Super Bright 436

Gene

Ly6a

Gene Alias

9804; locus A/E; Locus AE; Ly6; Ly67; Ly6a; Ly-6A.2; Ly-6A.2/Ly-6E.1; Ly6A/E; Ly-6A/E; ly6a-e; Ly6e; Ly-6E; Ly-6E.1; lymphocyte antigen 6 complex; lymphocyte antigen 6 complex, locus A; lymphocyte antigen 6 complex, locus E; lymphocyte antigen 6A-2/6E-1; Lymphocyte antigen 6E; RIG-E; Sca1; Sca-1; Sca-2; stem cell antigen 1; Stem cell antigen 2; TAP; T-cell-activating protein; thymic shared antigen 1; Tsa1; TSA-1

Host Species

Rat

Quantity

100 μg

Primary or Secondary

Primary

Target Species

Mouse

Product Type

Antibody

Isotype

IgG2a κ

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Description

  • Description: The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins
  • D7 reacts with both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid population and peripheral T and B cells
  • Non-activated splenocytes from Ly-6.2 strains including C57BL, SJL, 129, AKR, and others demonstrate higher frequency of D7-positive cells compared to the Ly-6.1 strains such as BALB/c, C3H, NZB and others
  • Sca-1 is expressed at high levels upon activation regardless of the Ly-6 haplotype
  • The Ly-6 family is involved in regulation and function of T cell activation
  • Sca-1 is a major phenotypic marker for mouse hematopoietic progenitor/stem cell subset
  • Applications Reported: This D7 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This D7 antibody has been tested by flow cytometric analysis of mouse bone marrow cells
  • This can be used at less than or equal to 0.25 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm
  • We recommend using a 450/50 bandpass filter, or equivalent
  • Stem Cell Antigen-1 (Sca-1) is a member of the lymphocyte antigen 6 (Ly-6) family
  • It is expressed on multipotent hematopoietic stem cells (HSC) and is used as a marker of HSC in mice
  • Sca-1 is used in combination with lineage depletion antibodies to identify murine HSC
  • Sca-1 can be found in fetal liver, adult bone marrow, and adult peripheral blood and spleen
  • Sca-1 may be involved in the regulation of B and T cell activation.

Compare Similar Items

Show Difference

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Invitrogen

62-598-182

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Antigen:
Ly-6A/E (Sca-1)

Classification:
Monoclonal

Concentration:
0.2 mg/mL

Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2

Gene Accession No.:
P05533, Q64253

Gene Symbols:
Ly6a, Ly6e

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
110454, 17069

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Flow Cytometry

Clone:
D7

Conjugate:
Super Bright 436

Gene:
Ly6a

Gene Alias:
9804; locus A/E; Locus AE; Ly6; Ly67; Ly6a; Ly-6A.2; Ly-6A.2/Ly-6E.1; Ly6A/E; Ly-6A/E; ly6a-e; Ly6e; Ly-6E; Ly-6E.1; lymphocyte antigen 6 complex; lymphocyte antigen 6 complex, locus A; lymphocyte antigen 6 complex, locus E; lymphocyte antigen 6A-2/6E-1; Lymphocyte antigen 6E; RIG-E; Sca1; Sca-1; Sca-2; stem cell antigen 1; Stem cell antigen 2; TAP; T-cell-activating protein; thymic shared antigen 1; Tsa1; TSA-1

Host Species:
Rat

Quantity:
100 μg

Primary or Secondary:
Primary

Target Species:
Mouse

Product Type:
Antibody

Isotype:
IgG2a κ

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Invitrogen

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Antigen:
CD274 (PD-L1, B7-H1)

Classification:
Monoclonal

Concentration:
0.2 mg/mL

Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2

Gene Accession No.:
Q9EP73

Gene Symbols:
Cd274

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
60533

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Flow Cytometry

Clone:
MIH5

Conjugate:
Super Bright 436

Gene:
Cd274

Gene Alias:
A530045L16Rik; B7 homolog 1; B7-H; B7h1; B7-H1; Cd274; CD274 antigen; CD274 molecule; PDCD1 ligand 1; Pdcd1l1; Pdcd1lg1; Pdl1; PD-L1; pdl-1; Programmed cell death 1 ligand 1; programmed death ligand 1; RGD1566211; sCD274; soluble CD274; soluble PD L1; sPD L1

Host Species:
Rat

Quantity:
25 μg

Primary or Secondary:
Primary

Target Species:
Mouse

Product Type:
Antibody

Isotype:
IgG2a λ

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Gene Accession No.:
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Gene Symbols:
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Purification Method:
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