Ly-6A/E (Sca-1) Monoclonal Antibody (D7), Super Bright™ 645, eBioscience™, Invitrogen™

Description

• Description: The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins
• D7 reacts with both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid population and peripheral T and B cells
• Non-activated splenocytes from Ly-6.2 strains including C57BL, SJL, 129, AKR, and others demonstrate higher frequency of D7-positive cells compared to the Ly-6.1 strains such as BALB/c, C3H, NZB and others
• Sca-1 is expressed at high levels upon activation regardless of the Ly-6 haplotype
• The Ly-6 family is involved in regulation and function of T cell activation
• Sca-1 is a major phenotypic marker for mouse hematopoietic progenitor/stem cell subset
• Applications Reported: This D7 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This D7 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm
• We recommend using a 660/20 bandpass filter
• Stem Cell Antigen-1 (Sca-1) is a member of the lymphocyte antigen 6 (Ly-6) family
• It is expressed on multipotent hematopoietic stem cells (HSC) and is used as a marker of HSC in mice
• Sca-1 is used in combination with lineage depletion antibodies to identify murine HSC
• Sca-1 can be found in fetal liver, adult bone marrow, and adult peripheral blood and spleen
• Sca-1 may be involved in the regulation of B and T cell activation.