CD284 (TLR4) Monoclonal Antibody (UT41), Super Bright™ 436, eBioscience™, Invitrogen™

Description

• Description: The UT41 monoclonal antibody was generated against BaF3 cells overexpressing mouse TLR4
• So far, at least twelve members of the Toll family have been identified
• This family of type I transmembrane protein is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor
• Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation
• TLR4 physically associates with another molecule called MD-2, and together with CD14, this complex is responsible for LPS recognition and signaling
• TLR4, which is approximately 100 kDa, is expressed by myeloid lineage cells
• The MTS510 monoclonal antibody co-immunoprecipitates MD-2 (∽30 kDa) and TLR4 (∽100 kDa), and preferentially reacts with TLR4 that is associated with MD-2
• In comparison, binding of the UT41 monoclonal antibody occurs with and without formation of the TLR4/MD-2 complex
• Applications Reported: This UT41 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This UT41 antibody has been tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells
• This may be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• TLR4 is member of the Toll like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity
• TLRs are highly conserved from Drosophila to humans and share structural and functional similarities
• This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes
• Mammalian cells respond to LPS by activating TLR4
• TLR4 belongs to the multi-protein complex of lipopolysaccharide (LPS) receptor, containing CD14, LY96 and TLR4, and is involved in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria
• TLR4 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity
• The various TLRs exhibit different patterns of expression
• Mutations in the TLR4 gene have been associated with differences in LPS responsiveness
• Also, several transcript variants of the TLR4 gene have been found, but the protein coding potential of most of them is uncertain
• TLR4 is expressed by peripheral blood monocytes and a small population of B-cells and is also expressed in human placenta
• Studies with TLR4-deficient mice indicate that the main ligand for TLR is lipopolysaccharide
• Consequently, these mice also showed increased susceptibility to Gram-negative sepsis.