HLA-A3 Monoclonal Antibody (GAP.A3), Super Bright™ 600, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody GAP.A3 reacts with the human major histocompatibility complex (MHC) class I molecule HLA-A3, which is a member of the HLA-A family
• HLA-A3 exists as two subtypes, HLA-A3.1 and HLA-A3.2, which differ at amino acid positions 152 and 156
• MHC class I molecules are expressed on all nucleated cells
• These molecules are highly polymorphic, cell surface glycoproteins that non-covalently associate with B2 microglobulin
• MHC Class I molecules present intracellular peptides on the cell surface and nonnative peptides are recognized by circulating cytotoxic T lymphocytes
• HLA-A3 is expressed by 15-25% of cancer patients and has been demonstrated to bind the tumor-associated antigen human telomerase reverse transcriptase (hTERT)
• Applications Reported: This GAP.A3 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This GAP.A3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at 5 μL (0.5 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm
• We recommend using a 610/20 bandpass filter
• HLA-A belongs to the HLA class I heavy chain paralogues
• This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin)
• The heavy chain is anchored in the membrane
• Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen
• They are expressed in nearly all cells
• The heavy chain is approximately 45 kDa and its gene contains 8 exons
• Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail
• Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule
• Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation
• Hundreds of HLA-A alleles have been described
• HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.