CD150 Monoclonal Antibody (mShad150), Super Bright™ 645, eBioscience™, Invitrogen™

Description

• Description: The eBioGHI/61 monoclonal antibody reacts with human CD163 (Haemoglobin Scavenger Receptor, M130), a member of the macrophage scavenger receptor family
• CD163 is expressed by tissue macrophages, circulating blood monocytes, and some reports suggest that it may also be expressed by haematopoietic progenitor cells
• CD163 functions as a high affinity scavenger receptor for the complex of haemoglobin and haptoglobin which forms to clear haemoglobin from the blood
• The high affinity for CD163 is specific for the haemoglobin-haptoglobin complex, whereas haemoglobin or haptoglobin display no to low affinity toward CD163
• Upon ligand binding, CD163 initiates signal transduction which leads to the production of IL-6 and IL-10
• Furthermore, it has been demonstrated that cytokines such as IL-6 and IL-10 can induce expression of CD163, whereas proinflammatory stimuli, such as LPS or TNF alpha, can cause shedding of a soluble form of CD163, whose function is unclear
• Applications Reported: This eBioGHI/61 (GHI/61) antibody has been reported for use in flow cytometric analysis
• Applications Tested: This eBioGHI/61 (GHI/61) antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
• This may be used at 5 μL (1.0 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• CD150 (SLAM, Signaling Lymphocyte Activation Molecule) belongs to the immunoglobulin gene superfamily, and is involved in T cell stimulation
• SLAM is constitutively expressed on peripheral blood memory T cells, T cell clones, immature thymocytes, and a proportion of B cells, and is rapidly induced on naive T cells after activation
• Activated B cells express the membrane bound form, the soluble form and the cytoplasmic isoforms of SLAM
• It is suggested that signalling through homophilic SLAM-SLAM binding during B to B and B to T cell interactions enhances the expansion and differentiation of activated B cells
• Reports suggest that the extracellular domain of CD150 is the receptor for the measles virus and acts as a co-activator on T cells and B cells
• Moreover, CD150 is thought to interact with SH2D1A and with PTPN11 via its cytoplasmic domain
• Mutations CD150 gene may be associated with X-linked lympho-proliferative disease (XLP).