64-480-182

F4/80 Monoclonal Antibody (BM8), Super Bright™ 645, eBioscience™, Invitrogen™

Manufacturer: Invitrogen

Select a Size

Pack Size SKU Availability Price
Each of 1 64-480-182-Each-of-1 In Stock ₹ 30,887.16

64-480-182 - Each of 1

₹ 30,887.16

In Stock

Quantity

1

Base Price: ₹ 30,887.16

GST (18%): ₹ 5,559.689

Total Price: ₹ 36,446.849

Antigen

F4/80

Classification

Monoclonal

Concentration

0.2 mg/mL

Formulation

PBS with BSA and 0.09% sodium azide; pH 7.2

Gene Accession No.

Q61549

Gene Symbols

ADGRE1

Purification Method

Affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

13733

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Form

Liquid

Applications

Flow Cytometry

Clone

BM8

Conjugate

Super Bright 645

Gene

ADGRE1

Gene Alias

ADGRE1; Adhesion G protein-coupled receptor E1; Adhesion G protein-coupled receptor E2; cell surface glycoprotein EMR1; cell surface glycoprotein F4/80; DD7A5-7; EGF-like module containing, mucin-like, hormone receptor-like 1; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-TM7; Emr1; EMR1 hormone receptor; F4/80; Gpf480; Ly71; lymphocyte antigen 71; TM7LN3

Host Species

Rat

Quantity

100μg

Primary or Secondary

Primary

Target Species

Mouse

Product Type

Antibody

Isotype

IgG2a κ

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Description

  • Description: The eBio299Arm monoclonal antibody is useful as an isotype control immunoglobulin
  • Applications Reported: This eBio299Arm antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This eBio299Arm antibody has been tested by flow cytometric analysis of mouse splenocytes
  • Use isotype control at the same concentration as experimental antibody
  • Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm
  • We recommend using a 660/20 bandpass filter
  • Please make sure that your instrument is capable of detecting this fluorochrome
  • When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product No
  • SB-4400) to minimize any non-specific polymer interactions
  • Please refer to the datasheet for Super Bright Staining Buffer for more information
  • Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
  • Please protect this vial and stained samples from light
  • Fixation: Samples can be stored in IC Fixation Buffer (Product No
  • 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No
  • 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
  • Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically
  • F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1)
  • Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation
  • The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen
  • F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node
  • The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.

Compare Similar Items

Show Difference

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Invitrogen

64-480-182

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Antigen:
F4/80

Classification:
Monoclonal

Concentration:
0.2 mg/mL

Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2

Gene Accession No.:
Q61549

Gene Symbols:
ADGRE1

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
13733

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Flow Cytometry

Clone:
BM8

Conjugate:
Super Bright 645

Gene:
ADGRE1

Gene Alias:
ADGRE1; Adhesion G protein-coupled receptor E1; Adhesion G protein-coupled receptor E2; cell surface glycoprotein EMR1; cell surface glycoprotein F4/80; DD7A5-7; EGF-like module containing, mucin-like, hormone receptor-like 1; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-TM7; Emr1; EMR1 hormone receptor; F4/80; Gpf480; Ly71; lymphocyte antigen 71; TM7LN3

Host Species:
Rat

Quantity:
100μg

Primary or Secondary:
Primary

Target Species:
Mouse

Product Type:
Antibody

Isotype:
IgG2a κ

Img

Invitrogen

64-504-042

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Antigen:
CD4

Classification:
Monoclonal

Concentration:
5 μL/Test

Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2

Gene Accession No.:
P33705

Gene Symbols:
CD4

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
403931

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Flow Cytometry

Clone:
YKIX302.9

Conjugate:
Super Bright 645

Gene:
CD4

Gene Alias:
Activation B7-1 antigen; B7; B7.1; B7-1; BB1; B-lymphocyte activation antigen B7; CD28LG; CD28LG1; CD4; CD4 antigen; CD4 antigen (p55); CD4 antigen p55; Cd4 molecule; CD4 precursor; CD4 receptor; CD4, allele 1; cd4a; CD4mut; CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); CD80 molecule; cell surface glycoprotein CD4; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; fCD4; L3T4; LAB7; Leu-3; Ly-4; lymphocyte antigen CD4; lymphocyte antigen CD4 precursor; membrane protein; p55; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4); T-lymphocyte activation antigen CD80; W3/25; W3/25 antigen

Host Species:
Rat

Quantity:
100 Tests

Primary or Secondary:
Primary

Target Species:
Canine

Product Type:
Antibody

Isotype:
IgG2a κ

Img

Fischer Scientific

64-511-0

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Antigen:
__

Classification:
__

Concentration:
__

Formulation:
__

Gene Accession No.:
__

Gene Symbols:
__

Purification Method:
__

Regulatory Status:
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Gene ID (Entrez):
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Content And Storage:
__

Form:
__

Applications:
__

Clone:
__

Conjugate:
__

Gene:
__

Gene Alias:
__

Host Species:
__

Quantity:
10 mg

Primary or Secondary:
__

Target Species:
__

Product Type:
__

Isotype:
__

Img

Fischer Scientific

64-515-0

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Antigen:
__

Classification:
__

Concentration:
__

Formulation:
__

Gene Accession No.:
__

Gene Symbols:
__

Purification Method:
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Regulatory Status:
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Content And Storage:
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Form:
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Applications:
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Clone:
__

Conjugate:
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Gene:
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Gene Alias:
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Host Species:
__

Quantity:
50 mg

Primary or Secondary:
__

Target Species:
__

Product Type:
__

Isotype:
__