CD196 (CCR6) Monoclonal Antibody (R6H1), Super Bright™ 436, eBioscience™, Invitrogen™

Description

• Description: This R6H1 monoclonal antibody reacts with CD196 (also known as CCR6), a seven transmembrane G protein-coupled receptor expressed on T, B, dendritic, natural killer, and Langerhans cells
•  This CC chemokine receptor uniquely binds MIP-3a/CCL20, a chemoattractant for dendritic cells, effector/memory T cells, and B cells
• CD196 is also involved in host defense and inflammation at epithelial sites
•  Furthermore, this receptor has been implicated in Th17 differentiation and CD4+FoxP3+ regulatory T cell development
• Applications Reported: This R6H1 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This R6H1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm
• We recommend using a 450/50 bandpass filter, or equivalent
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product No
• SB-4401) to minimize any non-specific polymer interactions
• CCR6 is a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors
• The gene is preferentially expressed by immature dendritic cells and memory T cells
• The ligand of this receptor is macrophage inflammatory protein 3 alpha (MIP-3 alpha)
• This receptor has been shown to be important for B-lineage maturation and antigen-driven B-cell differentiation, and it may regulate the migration and recruitment of dentritic and T cells during inflammatory and immunological responses
• Alternatively spliced transcript variants that encode the same protein have been described for this gene.