CD199 (CCR9) Monoclonal Antibody (BBC3M4), eFluor™ 660, eBioscience™, Invitrogen™

Description

• Description: This BBC3M4 monoclonal antibody reacts with human CD199 (CCR9), a 7 transmembrane spanning G-protein-coupled receptor
• CCR9 expression is regulated during thymic development and CCR9 deficiency results in mild impairment in thymocyte development
• In the periphery, CCR9 and alpha 4 beta 7 integrin are upregulated in response to dendritic cell-derived retinoic acid, conferring preferential trafficking to gut-associated tissues
• CCR9 is the receptor for CCL25 (TECK)
• Applications Reported: This BBC3M4 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This BBC3M4 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells
• This can be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• eFluor™ 660 is a replacement for Alexa Fluor™ 647
• eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm)
• Please make sure that your instrument is capable of detecting this fluorochome
• Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• The protein encoded by this gene is a member of the beta chemokine receptor family
• It is predicted to be a seven transmembrane protein similar to G protein-coupled receptors
• Chemokines and their receptors are key regulators of the thymocytes migration and maturation in normal and inflammation conditions
• The specific ligand of this receptor is CCL25
• It has been found that this gene is differentially expressed by T lymphocytes of small intestine and colon, suggested a role in the thymocytes recruitment and development that may permit functional specialization of immune responses in different segment of the gastrointestinal tract
• This gene is mapped to the chemokine receptor gene cluster region
• Two alternatively spliced transcript variants have been described.