CD199 (CCR9) Monoclonal Antibody (eBioCW-1.2 (CW-1.2)), eFluor™ 450, eBioscience™, Invitrogen™

Description

• Description: The eBioCW-1.2 monoclonal antibody reacts with mouse CCR9 (CD199), which is the receptor for thymus-expressed chemokine (TECK)
• CCR9 is a member of the G protein coupled receptor (GPCR) supergene family, and is involved in trafficking of T cell progenitors within the thymus
• CCR9 expression during thymocyte development commences at the double-negative (DN) 3 stage (CD4-CD8-CD25+CD44-), peaks in the double-positive (DP) stage (CD4+CD8+CD25-CD44-), and is down-regulated in commited CD4+ or CD8+ single-positive (SP) thymoyctes
• CCR9-deficient mice show a mild impairment in thymoycte development
• In the periphery, CCR9 is thought to be expressed on naive CD8+ T cells, but not on naive CD4+ T cells
• Applications Reported: This eBioCW-1.2 (CW-1.2) antibody has been reported for use in flow cytometric analysis
• Applications Tested: This eBioCW-1.2 (CW-1.2) antibody has been tested by flow cytometric analysis or mouse thymocytes
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• eFluor™ 450 is an alternative to Pacific Blue™
• eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm)
• The protein encoded by this gene is a member of the beta chemokine receptor family
• It is predicted to be a seven transmembrane protein similar to G protein-coupled receptors
• Chemokines and their receptors are key regulators of the thymocytes migration and maturation in normal and inflammation conditions
• The specific ligand of this receptor is CCL25
• It has been found that this gene is differentially expressed by T lymphocytes of small intestine and colon, suggested a role in the thymocytes recruitment and development that may permit functional specialization of immune responses in different segment of the gastrointestinal tract
• This gene is mapped to the chemokine receptor gene cluster region
• Two alternatively spliced transcript variants have been described.