CD127 Monoclonal Antibody (eBioRDR5), Super Bright™ 645, eBioscience™, Invitrogen™

Description

• Description: The mShad150 monoclonal antibody reacts with mouse CD150, an ∽70 kDa transmembrane glycoprotein also known as Signaling Lymphocyte Activation Molecule (SLAM)
• CD150 is expressed by T cells, in particular Th1, and B cells; this expression is rapidly upregulated upon activation
• Immature thymocytes and dendritic cells also express this antigen
• Signaling through SLAM in T cells induces proliferation and augmentation of the interferon-gamma response
• Furthermore, SLAM is thought to play a role in adhesion between the T cell and antigen-presenting cell
• The mShad150 antibody has been reported to also stain the CD150+CD244-CD48-CD41- population of pluripotent hematopoietic stem cells
• Applications Reported: This mShad150 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This mShad150 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This may be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm
• We recommend using a 660/20 bandpass filter
• CD127 (Interleukin-7, IL-7) is a glycoprotein involved in the regulation of lymphopoiesis
• The response of cells to CD127 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer
• CD127 consists of an alpha chain and a gamma chain
• The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules
• CD127 promotes the proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4+ and CD8+ T cells
• The biological effects of IL7 are mediated by the binding of IL7 to the specific cell surface receptor
• Diseases associated with CD127 dysfunction include severe combined immunodeficiency and T cell negative/B cell negative/NK positive severe combined immunodeficiency.