CD127 Monoclonal Antibody (eBioRDR5), eFluor™ 506, eBioscience™, Invitrogen™

Description

• Description: The rat IgG2b monoclonal antibody is useful as an isotype control immunoglobulin
• Applications Reported: This eB149/10H5 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This eB149/10H5 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse splenocytes
• Use the isotype control at the same concentration as the experimental antibody
• eFluor™ 506 can be excited with the violet laser line (405 nm) and emits at 506 nm
• We recommend using a 510/20 band pass filter, or equivalent
• Please make sure that your instrument is capable of detecting this fluorochrome
• Excitation: 405 nm; Emission: 506 nm; Laser: Violet Laser
• Filtration: 0.2 μm post-manufacturing filtered
• CD127 (Interleukin-7, IL-7) is a glycoprotein involved in the regulation of lymphopoiesis
• The response of cells to CD127 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer
• CD127 consists of an alpha chain and a gamma chain
• The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules
• CD127 promotes the proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4+ and CD8+ T cells
• The biological effects of IL7 are mediated by the binding of IL7 to the specific cell surface receptor
• Diseases associated with CD127 dysfunction include severe combined immunodeficiency and T cell negative/B cell negative/NK positive severe combined immunodeficiency.