7067878

Human TNF-alpha Antibody, R&D Systems™

Mouse Monoclonal Antibody has been used in 6 publications

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

TNF-alpha

Concentration

LYOPH

Applications

Flow Cytometry, Neutralization, CyTOF

Conjugate

Unconjugated

Host Species

Mouse

Target Species

Human

Gene Accession No.

P01375

Gene ID (Entrez)

7124

Immunogen

E. coli-derived recombinant human TNF-alpha Gly57-Leu233 (predicted) Accession # P01375

Primary or Secondary

Primary

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Test Specificity

Detects human TNF-alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 25-50% cross-reactivity with recombinant porcine TNF-alpha and recombinant rhesus macaque TNF-alpha is observed but no cross-reactivity with recombinant cotton rat TNF-alpha , recombinant rat TNF-alpha , recombinant human (rh) LT alpha 1/beta 2, rhLT alpha 2/beta 1, rhAPRIL, rhBAFF, rhEDA-A2, recombinant mouse EDA, rhFas Ligand, rhLIGHT, rhOX40 Ligand, rhTRAIL, rhTRANCE, rhTWEAK, or rhVEGI is observed.

Clone

6401

Dilution

Flow Cytometry 0.25 ug/10^6 cells, Neutralization 0.015-0.06 ug/mL, CyTOF-ready

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative

Gene Alias

APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha

Gene Symbols

TNF

Isotype

IgG1

Purification Method

Protein A or G purified from ascites

Content And Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

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Description

  • TNF-alpha Monoclonal specifically detects TNF-alpha in Human samples
  • It is validated for Flow Cytometry, Neutralization, CyTOF-ready.

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Antigen:
TNF-alpha

Concentration:
LYOPH

Applications:
Flow Cytometry, Neutralization, CyTOF

Conjugate:
Unconjugated

Host Species:
Mouse

Target Species:
Human

Gene Accession No.:
P01375

Gene ID (Entrez):
7124

Immunogen:
E. coli-derived recombinant human TNF-alpha Gly57-Leu233 (predicted) Accession # P01375

Primary or Secondary:
Primary

Reconstitution:
Reconstitute at 0.5 mg/mL in sterile PBS.

Test Specificity:
Detects human TNF-alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 25-50% cross-reactivity with recombinant porcine TNF-alpha and recombinant rhesus macaque TNF-alpha is observed but no cross-reactivity with recombinant cotton rat TNF-alpha , recombinant rat TNF-alpha , recombinant human (rh) LT alpha 1/beta 2, rhLT alpha 2/beta 1, rhAPRIL, rhBAFF, rhEDA-A2, recombinant mouse EDA, rhFas Ligand, rhLIGHT, rhOX40 Ligand, rhTRAIL, rhTRANCE, rhTWEAK, or rhVEGI is observed.

Clone:
6401

Dilution:
Flow Cytometry 0.25 ug/10^6 cells, Neutralization 0.015-0.06 ug/mL, CyTOF-ready

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative

Gene Alias:
APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha

Gene Symbols:
TNF

Isotype:
IgG1

Purification Method:
Protein A or G purified from ascites

Content And Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Img

Fischer Scientific

7067879

Mouse Monoclonal Antibody...


Antigen:
TNF-alpha

Concentration:
__

Applications:
Neutralization, Immunocytochemistry

Conjugate:
Unconjugated

Host Species:
Mouse

Target Species:
Human

Gene Accession No.:
P01375

Gene ID (Entrez):
7124

Immunogen:
__

Primary or Secondary:
Primary

Reconstitution:
__

Test Specificity:
Detects human TNF-alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 25-50% cross-reactivity with recombinant porcine TNF-alpha and recombinant rhesus macaque TNF-alpha is observed but no cross-reactivity with recombinant cotton rat TNF-alpha , recombinant rat TNF-alpha , recombinant human (rh) LT alpha 1/beta 2, rhLT alpha 2/beta 1, rhAPRIL, rhBAFF, rhEDA-A2, recombinant mouse EDA, rhFas Ligand, rhLIGHT, rhOX40 Ligand, rhTRAIL, rhTRANCE, rhTWEAK, or rhVEGI is observed.

Clone:
6401R

Dilution:
Neutralization 0.1-0.6 ug/mL, Immunocytochemistry 3-25 ug/mL

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Formulation:
__

Gene Alias:
APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha

Gene Symbols:
TNF

Isotype:
IgG1

Purification Method:
Protein A or G purified from cell culture supernatant

Content And Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

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7067882

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Antigen:
Complement Component C5a

Concentration:
__

Applications:
Western Blot, Neutralization, ELISA

Conjugate:
Unconjugated

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
P06684

Gene ID (Entrez):
727

Immunogen:
E. coli-derived recombinant mouse Complement Component C5a Asn679-Arg755 Accession # P06684

Primary or Secondary:
Primary

Reconstitution:
Reconstitute at 0.5 mg/mL in sterile PBS.

Test Specificity:
Detects mouse Complement Component C5a in Western blots. In Western blots, this antibody does not cross-react with recombinant mouse Complement Component C3d, recombinant human (rh) Complement Component C3a, or rhComplement Component C5a.

Clone:
295108

Dilution:
Western Blot 1 ug/mL, Neutralization 0.1-0.5 ug/mL, ELISA Capture (Matched Antibody Pair) 2-8 ug/mL

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative

Gene Alias:
C5, C5A, HE

Gene Symbols:
Hc

Isotype:
IgG2a

Purification Method:
Protein A or G purified from hybridoma culture supernatant

Content And Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

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7067883

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Antigen:
IL-10

Concentration:
__

Applications:
Western Blot, Neutralization

Conjugate:
Unconjugated

Host Species:
Mouse

Target Species:
Human

Gene Accession No.:
P22301

Gene ID (Entrez):
3586

Immunogen:
__

Primary or Secondary:
Primary

Reconstitution:
__

Test Specificity:
Detects human IL-10 in direct ELISAs.

Clone:
23738R

Dilution:
Western Blot 2 ug/mL, Neutralization 5 ug/mL

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Formulation:
__

Gene Alias:
CSIF, CSIFMGC126450, Cytokine synthesis inhibitory factor, GVHDS, IL10, IL10A, IL-10MGC126451, interleukin 10, interleukin-10, TGIF

Gene Symbols:
IL10

Isotype:
IgG2b

Purification Method:
Protein A or G purified from cell culture supernatant

Content And Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.