CD5 Monoclonal Antibody (YKIX322.3), PE, eBioscience™, Invitrogen™

Description

• Description: This YKIX322.3 monoclonal antibody reacts with canine CD5, also known as Ly-1
• CD5 is a 67-kDa type I transmembrane glycoprotein expressed on thymocytes and mature T cells
• Studies have demonstrated that NK cells in dogs also express CD5, but at lower levels
• In dogs, CD5 is not detectable on B cells or B cell lines
• Applications Reported: This YKIX322.3 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This YKIX322.3 antibody has been pre-titrated and tested by flow cytometric analysis of normal canine peripheral blood cells
• This can be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
• Filtration: 0.2 μm post-manufacturing filtered
• CD5 is a 67 kDa human T-lymphocyte single-chain transmembrane glycoprotein
• CD5 is present on all mature T-lymphocytes, on most of thymocytes and on many T-cell leukemias and lymphomas
• CD5 also reacts with a subpopulation of activated B-cells and may act as a receptor in regulating T-cell proliferation
• CD5 is found on 95% of thymocytes and 72% of peripheral blood lymphocytes
• In lymph nodes, the main reactivity is observed in T cell areas
• CD5 is expressed by many T cell leukemia, lymphomas, and activated T cells
• Diseases associated with CD5 dysfunction include thymus cancer and Richter's Syndrome.