Galectin 3 Monoclonal Antibody (eBioM3/38 (M3/38)), PE, eBioscience™, Invitrogen™

Description

• Description: The eBioM3/38 antibody reacts with mouse Galectin-3 (Mac-2)
• Galectins are a family of animal lectins which appear to exhibit a variety of biological functions
• Galectin 3 (aka LGALS3, galactose-specific soluble lectin 3, Mac-2, L-29) is one of the more extensively studied members of this family and is a 30 kDa beta-galactoside-binding protein
• Due to a C-terminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homo-oligomerized
• Galectin 3 is normally distributed in epithelia of many organs and various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells
• The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins
• This monoclonal antibody eBioM3/38 crossreacts to human Galectin-3 (LGALS3)
• Applications Reported: This eBioM3/38 (M3/38) antibody has been reported for use in flow cytometric analysis
• Applications Tested: This eBioM3/38 (M3/38) antibody has been tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal macrophages
• This can be used at less than or equal to 0.06 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Galactose-specific lectin which binds IgE
• May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration
• Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis
• In the nucleus: acts as a pre-mRNA splicing factor
• Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.