7068279

CD252 (OX40 Ligand) Monoclonal Antibody (RM134L), PE, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

CD252 (OX40 Ligand)

Concentration

0.2 mg/mL

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Alias

Ath1; Ath-1; atherosclerosis 1; CD134 ligand; CD134L; CD252; glycoprotein Gp34; GP34; OX40 antigen ligand; OX40 ligand; OX40L; OX-40L; OX4OL; TAX transcriptionally-activated glycoprotein 1; tax-transcriptionally activated glycoprotein 1 (34kD); tax-transcriptionally activated glycoprotein 1 ligand; TNFSF4; TNLG2B; tumor necrosis factor (ligand) superfamily member 4; tumor necrosis factor (ligand) superfamily, member 4; tumor necrosis factor ligand 2B; tumor necrosis factor ligand superfamily member 4; tumor necrosis factor superfamily member 4; TXGP1; Txgp1l

Gene Symbols

Tnfsf4

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Gene

Tnfsf4

Clone

RM134L

Applications

Flow Cytometry

Conjugate

PE

Host Species

Rat

Target Species

Mouse

Gene Accession No.

P43488

Gene ID (Entrez)

22164

Isotype

IgG2b κ

Purification Method

Affinity chromatography

Product Type

Antibody

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Description

  • Description: The RM134L monoclonal antibody reacts with mouse CD252 also known as OX-40 Ligand, a member of the TNF superfamily
  • OX-40L is induced on mouse splenic B cells stimulated with a combination of anti-IgM and anti-CD40
  • Neither resting nor activated mouse T cells express OX-40L
  • The interaction of OX-40 (CD134) with OX-40L has been implicated in T cell-dependent humoral response
  • RM134L inhibits the costimulatory activity of mouse OX-40L transfected cells for anti-CD3-stimulated T cell proliferation
  • Applications Reported: This RM134L antibody has been reported for use in flow cytometric analysis
  • Applications Tested: The RM134L antibody has been tested by flow cytometric analysis of activated mouse splenocytes and can be used at less than or equal to 0.5 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
  • Filtration: 0.2 μm post-manufacturing filtered
  • The tumor necrosis factor superfamily member TNFSF4 is a type II membrane bound, non-covalently linked homotrimeric protein
  • It is expressed on antigen presenting cells, such as dendritic cells and activated B-cells, and also on other cells such as vascular endothelial cells, mast cells, and natural killer cells
  • TNFSF4 signals specifically through the TNFRSF4 receptor, is expressed predominantly on CD4+T cells but also on certain activated CD8+T cells
  • TNFRSF4/TNFSF4 functions as a costimulatory signal, which is required for a productive interaction between antigen presenting cells and their target T-cells
  • It enhances cell proliferation and survival, and increases expression of RANTES, IL-2, IL-3, and IFNgamma
  • TNFRSF4/TNFSF4 signaling plays an important role in immunotolerance.

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Antigen:
CD252 (OX40 Ligand)

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
Ath1; Ath-1; atherosclerosis 1; CD134 ligand; CD134L; CD252; glycoprotein Gp34; GP34; OX40 antigen ligand; OX40 ligand; OX40L; OX-40L; OX4OL; TAX transcriptionally-activated glycoprotein 1; tax-transcriptionally activated glycoprotein 1 (34kD); tax-transcriptionally activated glycoprotein 1 ligand; TNFSF4; TNLG2B; tumor necrosis factor (ligand) superfamily member 4; tumor necrosis factor (ligand) superfamily, member 4; tumor necrosis factor ligand 2B; tumor necrosis factor ligand superfamily member 4; tumor necrosis factor superfamily member 4; TXGP1; Txgp1l

Gene Symbols:
Tnfsf4

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
Tnfsf4

Clone:
RM134L

Applications:
Flow Cytometry

Conjugate:
PE

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
P43488

Gene ID (Entrez):
22164

Isotype:
IgG2b κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Antigen:
iNOS

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
Hepatocyte NOS; hepatocytes; HEP-NOS; inducible nitric oxide synthase; inducible NO synthase; Inducible NOS; iNos; i-NOS; Inosl; MAC-NOS; macrophage NOS; nitric oxide synthase 2; nitric oxide synthase 2, inducible; nitric oxide synthase 2, inducible, macrophage; nitric oxide synthase 2A (inducible, hepatocytes); nitric oxide synthase, inducible; nitric oxide synthase, macrophage; nitric oxide synthase-inducible; NOS; NOS type II; NOS, type II; Nos2; Nos-2; Nos2a; NOS-II; OTTMUSP00000000202; peptidyl-cysteine S-nitrosylase NOS2

Gene Symbols:
Nos2

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
Nos2

Clone:
CXNFT

Applications:
Flow Cytometry

Conjugate:
PE

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
P29477

Gene ID (Entrez):
18126

Isotype:
IgG2a κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Antigen:
TER-119

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
Ly76; Ly-76; TER119

Gene Symbols:
Ly76

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
Ly76

Clone:
TER-119

Applications:
Flow Cytometry

Conjugate:
PE

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
0

Gene ID (Entrez):
104231

Isotype:
IgG2b κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Antigen:
AHR

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
Ah; Ah receptor; Ahh; Ahr; Ahre; AH-receptor; aromatic hydrocarbon receptor; aryl hydrocarbon receptor; aryl-hydrocarbon receptor; bHLHe76; Class E basic helix-loop-helix protein 76; dioxin receptor; In

Gene Symbols:
AHR

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
AHR

Clone:
4MEJJ

Applications:
Flow Cytometry

Conjugate:
PE

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
P30561

Gene ID (Entrez):
11622

Isotype:
IgG2a κ

Purification Method:
Affinity chromatography

Product Type:
Antibody