Phospho-p38 MAPK (Thr180, Tyr182) Monoclonal Antibody (4NIT4KK), PE, eBioscience™, Invitrogen™

Description

• Description: This 4NIT4KK monoclonal antibody recognizes human and mouse p38 mitogen-activated protein kinase (MAPK) when phosphorylated on T180/Y182
• p38 MAPK belongs to a family of conserved serine/threonine protein kinases that are phosphorylated and activated in response to numerous stress stimuli including TLR ligands (such as LPS), osmotic shock, heat shock, UV irradiation, and inflammatory cytokines
• There are four p38 MAPK members that include p38 alpha, p38 beta, p38 gamma, and p38 delta
• The primary activators of p38 MAPK are MKK3/4 and MKK6
• Several downstream targets of p38 MAPK have been identified including MK2/3, p53, ATF-2, and ETS1
• p38 MAPK can be negatively regulated by the chemical inhibitor SB203580
• Specificity of this 4NIT4KK clone was determined by ELISA, flow cytometry, and western blotting
• Applications Reported: This 4NIT4KK antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This 4NIT4KK antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells
• This can be used at 5 μL (0.06 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• The p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation, and other signals
• p38 MAPK is relatively inactive in the nonphosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motifs
• There are four isoforms of p38 MAPK, which differ in their tissue expression and affinity for upstream activators and downstream effectors
• When cells are exposed to tumor necrosis factor, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 occurs by phosphorylation
• Activated MAPK kinase-3/6 phosphorylate each residue of Thr180 and Tyr182 in p38 MAPK
• Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2 and other transcription factors through phosphorylation.