c-MAF Monoclonal Antibody (sym0F1), PE, eBioscience™, Invitrogen™

Description

• Description: The sym0F1 monoclonal antibody reacts with human and mouse c-Maf, a 42 kDa basic leucine zipper transcription factor shown to be involved in the neural, ocular and hematopoietic systems
• In hematopoietic cells, it was first shown to be crucial for IL-4 expression in Th2 and was the first transcription factor believed to be Th subset-specific
• More recent evidence shows that specific phospho-tyrosine residues lead to upregulation of IL-4
• c-Maf has also been shown to be important to differentiation and function in both Th17 and Tfh cells
• It drives expression of IL-21 in both cell types, while promoting expression of IL-23R in Th17 and CXCR5 in Tfh as well
• Sym0F1 does not cross-react with MafA or MafB
• Applications Reported: This sym0F1 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This sym0F1 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of Th17-polarized mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat
• 00-5523) and protocol
• Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online
• This can be used at 5 μL (0.06 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• c-maf is the cellular counterpart of oncogenic v-maf that belongs to the family of basic region leucine zipper domain transcription factors
• The leucine-zipper domain is involved in the interaction with LRPICD
• There are two forms of human c-maf mRNA, c-maf-long and c-maf-short
• It is identified in the genome of the acute transforming avian retrovirus AS42
• c-maf targets are IL-4 in Th2 cells, the crystalline genes in lens fiber cells, insulin gene in islet, p53 and L7 where it exerts its transcriptional role through binding to a Maf recognition element (MARE)
• It regulates Th2 differentiation and lineage-specific hematopoiesis
• c-maf is a transcription factor for IL-10 gene expression in LPS-activated macrophages
• Chromosomal aberration involving maf is found in some forms of multiple myeloma
• It is expressed in myeloma cell lines and resting monocytes/macrophages.