7068594

CD14 Monoclonal Antibody (61D3), FITC, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

CD14

Concentration

5 μL/Test

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Alias

CD 14; CD14; CD14 antigen; CD14 molecule; cd14 monocyte; lipopolysaccharide receptor; lipoprotein receptor; LPSR antibody; monocyte differentiation antigen CD14; Monocyte differentiation antigen CD14, membrane-bound form; Monocyte differentiation antigen CD14, urinary form; monocyte differentiation antigen CD14; LOW QUALITY PROTEIN: monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein; myeloid membrane glycoprotein precursor; sCD14; soluble CD14

Gene Symbols

Cd14

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Gene

Cd14

Clone

61D3

Applications

Flow Cytometry

Conjugate

FITC

Host Species

Mouse

Target Species

Human

Gene Accession No.

P08571

Gene ID (Entrez)

929

Isotype

IgG1 κ

Purification Method

Affinity chromatography

Product Type

Antibody

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Description

  • Description: The 61D3 monoclonal antibody reacts with human CD14, a 53-55 kDa GPI-linked glycoprotein
  • CD14 is expressed on monocytes, interfollicular macrophages and some dendritic cells
  • Complexes of LPS and LBP (LPS-Binding Protein) bind with high affinity to monocytes through the surface CD14
  • Applications Reported: The 61D3 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: The 61D3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
  • This can be used at 5 μL (1 μg) per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
  • Filtration: 0.2 μm post-manufacturing filtered
  • CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils
  • CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form
  • CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein
  • The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles
  • Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes
  • Alternative splicing results in multiple transcript variants encoding the same CD14 isoform
  • Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.

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7068594

Mouse Monoclonal Antibody...


Antigen:
CD14

Concentration:
5 μL/Test

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Alias:
CD 14; CD14; CD14 antigen; CD14 molecule; cd14 monocyte; lipopolysaccharide receptor; lipoprotein receptor; LPSR antibody; monocyte differentiation antigen CD14; Monocyte differentiation antigen CD14, membrane-bound form; Monocyte differentiation antigen CD14, urinary form; monocyte differentiation antigen CD14; LOW QUALITY PROTEIN: monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein; myeloid membrane glycoprotein precursor; sCD14; soluble CD14

Gene Symbols:
Cd14

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
Cd14

Clone:
61D3

Applications:
Flow Cytometry

Conjugate:
FITC

Host Species:
Mouse

Target Species:
Human

Gene Accession No.:
P08571

Gene ID (Entrez):
929

Isotype:
IgG1 κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Antigen:
CD16

Concentration:
5 μL/Test

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Alias:
4833442P21Rik; CD16; CD16-2; CD16A; CD16a antigen; CD16b; CD32; CD32 receptor 2; CDw32; cytolytic trigger molecule G7; Fc fragment of IgG intermediate affinity IV receptor; Fc fragment of IgG low affinity IIIa receptor; Fc fragment of IgG receptor IIIa; Fc fragment of IgG receptor IIIb; Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor; Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc fragment of IgG, low affinity IIIa, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor for (CD16); Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIb, receptor (CD16b); Fc gamma receptor III; Fc gamma receptor IIIa; Fc gamma receptor III-A; Fc gamma receptor IIIb; Fc gamma RIIIa; Fc gammaRIV; Fc receptor, IgG, low affinity III; Fc receptor, IgG, low affinity IV; Fc receptor-like 3; Fcg receptor III; FCG2; FCG3; Fc-gamma receptor III-2 (CD 16); Fc-gamma receptor IIIb (CD 16); Fc-gamma receptor IIIb (CD16); Fc-gamma RII-b; Fc-gamma RII-c; fc-gamma RIII; Fc-gamma RIIIa; Fc-gamma RIII-alpha; fc-gamma RIIIb; fc-gamma RIII-beta; Fc-gamma-RIIb; Fc-gamma-RIIc; FcgammaRIII; FcgammaRIII a.1; FcgammaRIII a.2; FcgammaRIII a.3; FcgammaRIIIA; FcgammaRIV; FCGR2; FCGR2A; FCGR2B; FCGR2C; FCGR3; Fcgr3a; FCGR3B; Fcgr4; FCGRIII; FcgRIV; FcR-10; FcRII-b; FcRII-c; FcRIII; FCRIIIA; FCRIIIb; Fcrl3; IGFR3; IgG Fc gamma receptor III; igG Fc receptor III; IgG Fc receptor III-1; igG Fc receptor III-2; IMD20; immunoglobulin G Fc receptor III; LOC100911825; Low affinity immunoglobulin gamma Fc region receptor III; low affinity immunoglobulin gamma Fc region receptor III-A; Low affinity immunoglobulin gamma Fc region receptor III-B; low affinity immunoglobulin gamma Fc region receptor III-like; Low affinity immunoglobulin gamma Fc region receptor IV; neutrophil-specific antigen NA; RP11-5K23.1; transmembrane receptor FcgammaRIII-X

Gene Symbols:
FCGR3A, FCGR3B

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
__

Clone:
eBioCB16 (CB16)

Applications:
Flow Cytometry

Conjugate:
FITC

Host Species:
Mouse

Target Species:
Human

Gene Accession No.:
O75015, P08637

Gene ID (Entrez):
2214, 2215

Isotype:
IgG1 κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Antigen:
CD18 (LFA-1 beta)

Concentration:
0.5 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
2E6; AI528527; antigen CD18; B-2 integrin; CD18; CD18 leukocyte adhesion molecule; CD18 subunit; cell surface adhesion glycoprotein (LFA-1/CR3/P150,959 beta subunit precursor); cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; complement component 3 receptor 3 and 4 subunit; complement receptor C3 beta-subunit; complement receptor C3 subunit beta; integrin beta 2; integrin beta chain, beta 2; integrin beta-2; integrin subunit beta 2; integrin, beta 2; integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1; integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1; macrophage antigen 1 (mac-1) beta subunit); integrin, beta 2 (antigen CD18 subunit (p95), lymphocyte function-associated antigen 1, integrin B2); integrin, beta 2 (complement component 3 receptor 3 and 4 subunit); ITGB2; LAD; LCAMB; Leu-CAM receptor; leukocyte cell adhesion molecule CD18; leukocyte surface protein; leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C; Lfa1; LFA-1; lymphocyte function associated antigen 1; MAC-1; Mac-1 beta; macrophage antigen 1 (mac-1) beta subunit); macrophage antigen-1 beta; MF17; MFI7

Gene Symbols:
ITGB2

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
ITGB2

Clone:
M18/2

Applications:
Flow Cytometry

Conjugate:
FITC

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
P11835

Gene ID (Entrez):
16414

Isotype:
IgG2a κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Antigen:
CD19

Concentration:
0.5 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
AW495831; B4; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Cd19; CD19 antigen; CD19 molecule; CVID3; differentiation antigen CD19; Leu-12; T-cell surface antigen Leu-12

Gene Symbols:
Cd19

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
Cd19

Clone:
MB19-1

Applications:
Flow Cytometry

Conjugate:
FITC

Host Species:
Mouse

Target Species:
Mouse

Gene Accession No.:
P25918

Gene ID (Entrez):
12478

Isotype:
IgA κ

Purification Method:
Affinity chromatography

Product Type:
Antibody