7068721

CD1d Monoclonal Antibody (1B1), PE, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

CD1d

Concentration

0.2 mg/mL

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Alias

AI747460; Antigen-presenting glycoprotein CD1d; antigen-presenting glycoprotein CD1d1; Cd1; CD1.1; CD1A; Cd1d; CD1D antigen; CD1D antigen, d polypeptide; CD1d molecule; CD1d.1; Cd1d1; CD1d1 antigen; CD1d1 molecule; differentiation antigen CD1-alpha-3; HMC class I antigen-like glycoprotein CD1D; Ly-38; R3; R3G1; T-cell surface glycoprotein CD1d; T-cell surface glycoprotein CD1d1; thymocyte antigen CD1D

Gene Symbols

Cd1d1

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Gene

Cd1d1

Clone

1B1

Applications

Flow Cytometry

Conjugate

PE

Host Species

Rat

Target Species

Mouse

Gene Accession No.

P11609

Gene ID (Entrez)

12479

Isotype

IgG2b κ

Purification Method

Affinity chromatography

Product Type

Antibody

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Description

  • Description: The 1B1 monoclonal antibody reacts with mouse CD1d, a 48 kDa glycoprotein with structural homology to MHC class I molecules
  • While similar to MHC Class I, CD1d associates with beta2m, functionally CD1d is similar to MHC Class II
  • 1B1 detects CD1d at varying levels on mouse leukocytes
  • 1B1 detects beta2m associated CD1d
  • Applications Reported: The 1B1 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: The 1B1 antibody has been tested by flow cytometric analysis of mouse splenocytes
  • This can be used at less than or equal to 0.125 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
  • Filtration: 0.2 μm post-manufacturing filtered
  • CD1d is a heavy chain associated with Beta 2 microglobulin on cortical thymocytes
  • Beta 2 microglobulin independent expression of CD1d has also been demonstrated on human intestinal epithelial cells
  • CD1d is the sole group 2 member of the CD1 family of major histocompatibility (MHC) like glycoproteins
  • The CD1d gene encodes a divergent member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin
  • The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells
  • The human genome contains five CD1 family genes organized in a cluster on chromosome 1
  • The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands
  • The protein encoded by the CD1d gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail
  • Diseases associated with CD1D include Mycobacterium Malmoense and Autoimmune Disease.

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Antigen:
CD1d

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
AI747460; Antigen-presenting glycoprotein CD1d; antigen-presenting glycoprotein CD1d1; Cd1; CD1.1; CD1A; Cd1d; CD1D antigen; CD1D antigen, d polypeptide; CD1d molecule; CD1d.1; Cd1d1; CD1d1 antigen; CD1d1 molecule; differentiation antigen CD1-alpha-3; HMC class I antigen-like glycoprotein CD1D; Ly-38; R3; R3G1; T-cell surface glycoprotein CD1d; T-cell surface glycoprotein CD1d1; thymocyte antigen CD1D

Gene Symbols:
Cd1d1

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
Cd1d1

Clone:
1B1

Applications:
Flow Cytometry

Conjugate:
PE

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
P11609

Gene ID (Entrez):
12479

Isotype:
IgG2b κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Antigen:
CD1a

Concentration:
5 μL/Test

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Alias:
CD1; CD1 antigen; CD1.1; CD1A; CD1A antigen, a polypeptide; CD1a molecule; cd1a1; CD1A1 antigen; cluster of differentiation 1 A; cortical thymocyte antigen CD1A; differentiation antigen CD1-alpha-3; epidermal dendritic cell marker CD1a; FCB6; HTA1; hTa1 thymocyte antigen; R4; T6; T-cell surface antigen T6/Leu-6; T-cell surface glycoprotein CD1a

Gene Symbols:
CD1A

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
CD1A

Clone:
SK9

Applications:
Flow Cytometry

Conjugate:
PE

Host Species:
Mouse

Target Species:
Human

Gene Accession No.:
P06126

Gene ID (Entrez):
909

Isotype:
IgG2b

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Antigen:
CD2

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
Cd2; CD2 antigen; CD2 antigen (p50), sheep red blood cell receptor; CD2 molecule; CD2R; cluster of differentiation 2; Erythrocyte receptor; FLJ46032; LFA2; LFA-2; LFA-3 receptor; Ly37; Ly-37; lymphocyte antigen 37; OX34; OX-34; OX-34 antigen; OX-45 surface antigen homolog to human T lymphocyte CD2 antigen; Rosette receptor; SRBC; surface immunoglobulin superfamily molecule; T11; T-cell surface antigen CD2; T-cell surface antigen T11/Leu-5

Gene Symbols:
CD2

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
CD2

Clone:
RM2-5

Applications:
Flow Cytometry

Conjugate:
PE

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
P08920

Gene ID (Entrez):
12481

Isotype:
IgG2b λ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Antigen:
CD2

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
Cd2; CD2 antigen; CD2 antigen (p50), sheep red blood cell receptor; CD2 molecule; CD2R; cluster of differentiation 2; Erythrocyte receptor; FLJ46032; LFA2; LFA-2; LFA-3 receptor; Ly37; Ly-37; lymphocyte antigen 37; OX34; OX-34; OX-34 antigen; OX-45 surface antigen homolog to human T lymphocyte CD2 antigen; Rosette receptor; SRBC; surface immunoglobulin superfamily molecule; T11; T-cell surface antigen CD2; T-cell surface antigen T11/Leu-5

Gene Symbols:
CD2

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
CD2

Clone:
RM2-5

Applications:
Flow Cytometry

Conjugate:
PE

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
P08920

Gene ID (Entrez):
12481

Isotype:
IgG2b λ

Purification Method:
Affinity chromatography

Product Type:
Antibody