7068726

CD2 Monoclonal Antibody (RM2-5), PE, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

CD2

Concentration

0.2 mg/mL

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Alias

Cd2; CD2 antigen; CD2 antigen (p50), sheep red blood cell receptor; CD2 molecule; CD2R; cluster of differentiation 2; Erythrocyte receptor; FLJ46032; LFA2; LFA-2; LFA-3 receptor; Ly37; Ly-37; lymphocyte antigen 37; OX34; OX-34; OX-34 antigen; OX-45 surface antigen homolog to human T lymphocyte CD2 antigen; Rosette receptor; SRBC; surface immunoglobulin superfamily molecule; T11; T-cell surface antigen CD2; T-cell surface antigen T11/Leu-5

Gene Symbols

CD2

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Gene

CD2

Clone

RM2-5

Applications

Flow Cytometry

Conjugate

PE

Host Species

Rat

Target Species

Mouse

Gene Accession No.

P08920

Gene ID (Entrez)

12481

Isotype

IgG2b λ

Purification Method

Affinity chromatography

Product Type

Antibody

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Description

  • Description: The RM2-5 monoclonal antibody reacts with the mouse CD2 molecule, an approximately 50-55 kDa cell surface receptor expressed by all mouse lymphocytes
  • Expression of the CD2 antigen in the mouse differs from that of the human in that B lineage cells in the mouse from the pre-B cell stage to mature B cells express CD2
  • CD2 is a ligand for CD48 in the mouse and is involved in adhesion and activation of T cells
  • Applications Reported: The RM2-5 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This RM2-5 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells
  • This can be used at less than or equal to 0.25 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
  • Filtration: 0.2 μm post-manufacturing filtered
  • CD2 (LFA-2) is a monomeric surface antigen (MW range 45-58 kDa) of the human T-lymphocyte lineage that is expressed on all peripheral blood T cells
  • CD2 is one of the earliest T-cell markers, being present on more than 95% of thymocytes and it is also found on some natural killer cells, but not on B lymphocytes
  • Monoclonal antibodies directed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or is closely associated with it
  • The interaction between CD2 and CD58 stabilizes adhesion between T cells and antigen presenting or target cells
  • Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion
  • Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58
  • Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion
  • CD2 deficiency reduces intestinal inflammation and helps to control infection
  • Diseases associated with CD2 dysfunction include penis squamous cell carcinoma and immune deficiency due to the absence of the thymus.

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Antigen:
CD2

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
Cd2; CD2 antigen; CD2 antigen (p50), sheep red blood cell receptor; CD2 molecule; CD2R; cluster of differentiation 2; Erythrocyte receptor; FLJ46032; LFA2; LFA-2; LFA-3 receptor; Ly37; Ly-37; lymphocyte antigen 37; OX34; OX-34; OX-34 antigen; OX-45 surface antigen homolog to human T lymphocyte CD2 antigen; Rosette receptor; SRBC; surface immunoglobulin superfamily molecule; T11; T-cell surface antigen CD2; T-cell surface antigen T11/Leu-5

Gene Symbols:
CD2

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
CD2

Clone:
RM2-5

Applications:
Flow Cytometry

Conjugate:
PE

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
P08920

Gene ID (Entrez):
12481

Isotype:
IgG2b λ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Antigen:
CD3

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
4930549J05Rik; A430104F18Rik; AI504783; antigen CD3D, delta polypeptide (TiT3 complex); antigen CD3E, epsilon polypeptide (TiT3 complex); antigen CD3G, gamma polypeptide; antigen CD3Z, zeta polypeptide; AW552088; Cd247; CD247 antigen; CD247 antigen, zeta subunit; Cd247 molecule; CD3; CD3 antigen delta chain; CD3 antigen delta polypeptide; CD3 antigen gamma chain; CD3 antigen, delta polypeptide; CD3 antigen, delta subunit; CD3 antigen, epsilon polypeptide; CD3 antigen, gamma polypeptide; CD3 antigen, zeta polypeptide; CD3 delta; CD3 epsilon; CD3 epsilon chain; CD3 epsilon subunit; CD3 epsilon subunit precursor; CD3 gamma-chain; CD3 glycoprotein; CD3 glycoprotein precursor; CD3 molecule delta polypeptide; CD3 molecule, delta; CD3 molecule, epsilon; CD3 molecule, epsilon polypeptide; CD3 molecule, gamma; CD3 molecule, gamma polypeptide; CD3 protein; CD3 TCR complex; CD3 type I transmembrane glycoprotein; CD3 type I transmembrane glycoprotein precursor; CD3 zeta chain; Cd3d; CD3D antigen delta; CD3D antigen, delta polypeptide (TiT3 complex); CD3d molecule; CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; Cd3e; CD3E antigen, epsilon polypeptide; CD3E antigen, epsilon polypeptide (TiT3 complex); CD3e molecule; CD3e molecule, epsilon (CD3-TCR complex); CD3epsilon; CD3-epsilon; Cd3-eta; Cd3g; CD3G antigen, gamma polypeptide; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule; CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3-GAMMA; Cd3h; CD3Q; Cd3z; CD3Z antigen, zeta polypeptide (TiT3 complex); Cd3zeta; Cd3-zeta; CD3zeta chain; CD3-zeta/eta; Ctg3; Ctg-3; FLJ18683; IMD17; IMD18; IMD19; IMD25; Leu-4; OKT3, delta chain; T cell antigen receptor complex epsilon subunit of T3; T3/TCR complex; T3d; T3e; T3g; T3Z; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor CD3 epsilon chain; T-cell receptor CD3 epsilon subunit; T-cell receptor CD3 subunit zeta; T-cell receptor CD3, subunit zeta; T-cell receptor T3 delta chain; T-cell receptor T3 eta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell receptor zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T-cell surface protein; TcR CD3 delta-chain; TcR CD3 gamma-chain; TCR zeta chain; TCR zeta chain subunit; TCRE; Tcrk; TCRZ; TCRzeta; TiT3 complex; type I transmembrane protein; T-cell surface molecule CD3

Gene Symbols:
Cd247, Cd3d, CD3E, CD3g

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
Cd3d

Clone:
eBioG4.18 (G4.18)

Applications:
Flow Cytometry

Conjugate:
PE

Host Species:
Mouse

Target Species:
Rat

Gene Accession No.:
P19377, Q64159

Gene ID (Entrez):
25300, 25710, 300678, 315609

Isotype:
IgG3 κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Antigen:
CD3e

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
4930549J05Rik; A430104F18Rik; AI504783; antigen CD3D, delta polypeptide (TiT3 complex); antigen CD3E, epsilon polypeptide (TiT3 complex); antigen CD3G, gamma polypeptide; antigen CD3Z, zeta polypeptide; AW552088; Cd247; CD247 antigen; CD247 antigen, zeta subunit; Cd247 molecule; CD3; CD3 antigen delta chain; CD3 antigen delta polypeptide; CD3 antigen gamma chain; CD3 antigen, delta polypeptide; CD3 antigen, delta subunit; CD3 antigen, epsilon polypeptide; CD3 antigen, gamma polypeptide; CD3 antigen, zeta polypeptide; CD3 delta; CD3 epsilon; CD3 epsilon chain; CD3 epsilon subunit; CD3 epsilon subunit precursor; CD3 gamma-chain; CD3 glycoprotein; CD3 glycoprotein precursor; CD3 molecule delta polypeptide; CD3 molecule, delta; CD3 molecule, epsilon; CD3 molecule, epsilon polypeptide; CD3 molecule, gamma; CD3 molecule, gamma polypeptide; CD3 protein; CD3 TCR complex; CD3 type I transmembrane glycoprotein; CD3 type I transmembrane glycoprotein precursor; CD3 zeta chain; Cd3d; CD3D antigen delta; CD3D antigen, delta polypeptide (TiT3 complex); CD3d molecule; CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; Cd3e; CD3E antigen, epsilon polypeptide; CD3E antigen, epsilon polypeptide (TiT3 complex); CD3e molecule; CD3e molecule, epsilon (CD3-TCR complex); CD3epsilon; CD3-epsilon; Cd3-eta; Cd3g; CD3G antigen, gamma polypeptide; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule; CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3-GAMMA; Cd3h; CD3Q; Cd3z; CD3Z antigen, zeta polypeptide (TiT3 complex); Cd3zeta; Cd3-zeta; CD3zeta chain; CD3-zeta/eta; Ctg3; Ctg-3; FLJ18683; IMD17; IMD18; IMD19; IMD25; Leu-4; OKT3, delta chain; T cell antigen receptor complex epsilon subunit of T3; T3/TCR complex; T3d; T3e; T3g; T3Z; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor CD3 epsilon chain; T-cell receptor CD3 epsilon subunit; T-cell receptor CD3 subunit zeta; T-cell receptor CD3, subunit zeta; T-cell receptor T3 delta chain; T-cell receptor T3 eta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell receptor zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T-cell surface protein; TcR CD3 delta-chain; TcR CD3 gamma-chain; TCR zeta chain; TCR zeta chain subunit; TCRE; Tcrk; TCRZ; TCRzeta; TiT3 complex; type I transmembrane protein; T-cell surface molecule CD3

Gene Symbols:
Cd247, Cd3d, CD3E, CD3g

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
__

Clone:
145-2C11

Applications:
Flow Cytometry

Conjugate:
PE

Host Species:
Armenian Hamster

Target Species:
Mouse

Gene Accession No.:
P04235, P11942, P22646, P24161

Gene ID (Entrez):
12500, 12501, 12502, 12503

Isotype:
IgG

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Antigen:
CD3e

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
4930549J05Rik; A430104F18Rik; AI504783; antigen CD3D, delta polypeptide (TiT3 complex); antigen CD3E, epsilon polypeptide (TiT3 complex); antigen CD3G, gamma polypeptide; antigen CD3Z, zeta polypeptide; AW552088; Cd247; CD247 antigen; CD247 antigen, zeta subunit; Cd247 molecule; CD3; CD3 antigen delta chain; CD3 antigen delta polypeptide; CD3 antigen gamma chain; CD3 antigen, delta polypeptide; CD3 antigen, delta subunit; CD3 antigen, epsilon polypeptide; CD3 antigen, gamma polypeptide; CD3 antigen, zeta polypeptide; CD3 delta; CD3 epsilon; CD3 epsilon chain; CD3 epsilon subunit; CD3 epsilon subunit precursor; CD3 gamma-chain; CD3 glycoprotein; CD3 glycoprotein precursor; CD3 molecule delta polypeptide; CD3 molecule, delta; CD3 molecule, epsilon; CD3 molecule, epsilon polypeptide; CD3 molecule, gamma; CD3 molecule, gamma polypeptide; CD3 protein; CD3 TCR complex; CD3 type I transmembrane glycoprotein; CD3 type I transmembrane glycoprotein precursor; CD3 zeta chain; Cd3d; CD3D antigen delta; CD3D antigen, delta polypeptide (TiT3 complex); CD3d molecule; CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; Cd3e; CD3E antigen, epsilon polypeptide; CD3E antigen, epsilon polypeptide (TiT3 complex); CD3e molecule; CD3e molecule, epsilon (CD3-TCR complex); CD3epsilon; CD3-epsilon; Cd3-eta; Cd3g; CD3G antigen, gamma polypeptide; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule; CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3-GAMMA; Cd3h; CD3Q; Cd3z; CD3Z antigen, zeta polypeptide (TiT3 complex); Cd3zeta; Cd3-zeta; CD3zeta chain; CD3-zeta/eta; Ctg3; Ctg-3; FLJ18683; IMD17; IMD18; IMD19; IMD25; Leu-4; OKT3, delta chain; T cell antigen receptor complex epsilon subunit of T3; T3/TCR complex; T3d; T3e; T3g; T3Z; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor CD3 epsilon chain; T-cell receptor CD3 epsilon subunit; T-cell receptor CD3 subunit zeta; T-cell receptor CD3, subunit zeta; T-cell receptor T3 delta chain; T-cell receptor T3 eta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell receptor zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T-cell surface protein; TcR CD3 delta-chain; TcR CD3 gamma-chain; TCR zeta chain; TCR zeta chain subunit; TCRE; Tcrk; TCRZ; TCRzeta; TiT3 complex; type I transmembrane protein; T-cell surface molecule CD3

Gene Symbols:
Cd247, Cd3d, CD3E, CD3g

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
CD3g

Clone:
eBio500A2 (500A2)

Applications:
Flow Cytometry

Conjugate:
PE

Host Species:
Syrian Hamster

Target Species:
Mouse

Gene Accession No.:
P04235, P11942, P22646, P24161

Gene ID (Entrez):
12500, 12501, 12502, 12503

Isotype:
IgG

Purification Method:
Affinity chromatography

Product Type:
Antibody