7068606

CD23 Monoclonal Antibody (B3B4), FITC, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

CD23

Concentration

0.5 mg/mL

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Alias

BLAST-2; CD23; CD23 antigen; CD23A; CLEC4J; C-type lectin domain family 4 member J; C-type lectin domain family 4, member J; Fc epsilon receptor II; FC epsilon RII; Fc fragment of IgE receptor II; Fc fragment of IgE, low affinity II, receptor for (CD23); Fc receptor alpha multiple ligand receptor; Fc receptor, IgE, low affinity II, alpha polypeptide; Fcalpha muR; Fcalpha RII; Fce2; fc-epsilon-RII; FCER2; Fcer2a; FceRII; IGEBF; Immunoglobulin E-binding factor; immunoglobulin epsilon-chain; low affinity immunoglobulin epsilon Fc receptor; Low affinity immunoglobulin epsilon Fc receptor membrane-bound form; Low affinity immunoglobulin epsilon Fc receptor soluble form; low-affinity IgE receptor; Ly-42; lymphocyte IgE receptor

Gene Symbols

Fcer2a

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Gene

Fcer2a

Clone

B3B4

Applications

Flow Cytometry

Conjugate

FITC

Host Species

Rat

Target Species

Mouse

Gene Accession No.

P20693

Gene ID (Entrez)

14128

Isotype

IgG2a κ

Purification Method

Affinity chromatography

Product Type

Antibody

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Description

  • Description: The B3B4 monoclonal antibody reacts with mouse CD23, a 45 kDa type II transmembrane glycoprotein
  • CD23 is expressed on resting conventional B cells, and its expression is modulated upon B-cell activation
  • B-1 cell lineage (CD5+ B cells) does not express CD23
  • Soluble forms of the antigen have been reported to be biologically active
  • CD23 is a low affinity receptor for IgE and is thought to play a role in the regulation of IgE response and B-cell activation
  • CD21 is thought to bind to CD23
  • Applications Reported: The B3B4 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: The B3B4 antibody has been tested by flow cytometric analysis of mouse splenocytes
  • This can be used at less than or equal to 0.5 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
  • Filtration: 0.2 μm post-manufacturing filtered
  • CD23 is a 45 kDa glycoprotein which is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts
  • The CD23 molecule is identical to the low affinity IgE receptor found on B cells
  • Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic lymphocyctic leukaemia and some cases of centroblastic/centrocytic lymphoma
  • CD23 is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts
  • Functionally, CD23 is involved in B cell growth and differentiation, and IgE production
  • Further, CD23 has a soluble form that is a potent mitogenic factor
  • Diseases associated with CD23 dysfunction include chronic conjunctivitis and chronic lymphocytic leukemia.

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Antigen:
CD23

Concentration:
0.5 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
BLAST-2; CD23; CD23 antigen; CD23A; CLEC4J; C-type lectin domain family 4 member J; C-type lectin domain family 4, member J; Fc epsilon receptor II; FC epsilon RII; Fc fragment of IgE receptor II; Fc fragment of IgE, low affinity II, receptor for (CD23); Fc receptor alpha multiple ligand receptor; Fc receptor, IgE, low affinity II, alpha polypeptide; Fcalpha muR; Fcalpha RII; Fce2; fc-epsilon-RII; FCER2; Fcer2a; FceRII; IGEBF; Immunoglobulin E-binding factor; immunoglobulin epsilon-chain; low affinity immunoglobulin epsilon Fc receptor; Low affinity immunoglobulin epsilon Fc receptor membrane-bound form; Low affinity immunoglobulin epsilon Fc receptor soluble form; low-affinity IgE receptor; Ly-42; lymphocyte IgE receptor

Gene Symbols:
Fcer2a

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
Fcer2a

Clone:
B3B4

Applications:
Flow Cytometry

Conjugate:
FITC

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
P20693

Gene ID (Entrez):
14128

Isotype:
IgG2a κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Antigen:
CD24

Concentration:
__

Classification:
Monoclonal

Form:
__

Regulatory Status:
RUO

Formulation:
__

Gene Alias:
__

Gene Symbols:
__

Primary or Secondary:
Primary

Content And Storage:
__

Gene:
__

Clone:
30-F1

Applications:
Flow Cytometry

Conjugate:
FITC

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
__

Gene ID (Entrez):
__

Isotype:
IgG2c κ

Purification Method:
__

Product Type:
__

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Antigen:
CD24

Concentration:
0.5 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
CD24; CD24 antigen; CD24 antigen (small cell lung carcinoma cluster 4 antigen); CD24 molecule; CD24A; CD24a antigen; cluster of differentiation 24; FLJ22950; FLJ43543; heat stable antigen; heat-stable antigen; HSA; Ly-52; Lymphocyte antigen 52; M1/69-J11D heat stable antigen; MGC75043; Nectadrin; nectadrin heat stable antigen; R13-Ag; Signal transducer CD24; Small cell lung carcinoma cluster 4 antigen; X62 heat stable antigen

Gene Symbols:
Cd24a

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
Cd24a

Clone:
M1/69

Applications:
Flow Cytometry

Conjugate:
FITC

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
P24807

Gene ID (Entrez):
12484

Isotype:
IgG2b κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Antigen:
CD27

Concentration:
0.5 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
CD antigen 27; CD27; CD27 antigen; CD27 molecule; CD27L receptor; LPFS2; S152; S152. LPFS2; sCD27; soluble CD27; T cell activation antigen S152; T14; T-cell activation antigen CD27; TNFRSF7; TNFSF7; Tp55; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7

Gene Symbols:
CD27

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
CD27

Clone:
LG.7F9

Applications:
Flow Cytometry

Conjugate:
FITC

Host Species:
Armenian Hamster

Target Species:
Human, Mouse, Rat

Gene Accession No.:
P26842, P41272

Gene ID (Entrez):
21940, 500318, 939

Isotype:
IgG

Purification Method:
Affinity chromatography

Product Type:
Antibody