CD32b Monoclonal Antibody (AT130-2), PE, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody AT130-2 reacts with mouse CD32b, also known as Fc-gamma receptor IIb
• CD32b is the only inhibitory member of the Fc-gamma receptor family
• Immune complex engagement of CD32b results in the phosphorylation of its intracellular ITIM motif
• This engagement serves to attenuate B cell receptor (BCR) signaling and other activating signals, reduces proliferation, reduces antibody production, and may also deliver apoptotic signals
• CD32b is the only Fc-gamma receptor expressed by B cells
• CD32b is also expressed on monocytes, dendritic cells, neutrophils, eosinophils and basophils, as well as B cell progenitors and plasma cells
• AT130-2 has been reported to have agonist activity
• Applications Reported: This AT130-2 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This AT130-2 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
• Filtration: 0.2 μm post-manufacturing filtered
• This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells
• The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes
• Alternative splicing results in multiple transcript variants.