7069222

CD279 (PD-1) Monoclonal Antibody (J43), Biotin, eBioscience™, Invitrogen™

Armenian Hamster Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

CD279 (PD-1)

Concentration

0.5 mg/mL

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Gene Accession No.

Q02242

Gene Symbols

Pdcd1

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Gene

Pdcd1

Clone

J43

Applications

Flow Cytometry

Conjugate

Biotin

Host Species

Armenian Hamster

Target Species

Mouse

Gene ID (Entrez)

18566

Isotype

IgG

Purification Method

Affinity chromatography

Product Type

Antibody

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Description

  • Description: The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily
  • PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice
  • PD-1 is expressed mainly on activated T and B lymphocytes
  • Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC)
  • Evidence reported to date suggests overlapping functions for these ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells
  • It is reported that J43 inhibits the binding of mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1/BHK transfected cells
  • When administrated in vivo, both intact and Fab of J43 are reported to enhance contact hypersensitivity and exacerbate acute GVHD similar to transfer of PD-1-deficient cells
  • Injection of J43 also exacerbates EAE and NOD diabetes as do specific antibodies to mouse PD-L1 and PD-L2
  • Applications Reported: The J43 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: The J43 antibody has been tested by flow cytometric analysis of Con A-simulated mouse splenocytes and mouse PD-1 transfected cells
  • This can be used at less than or equal to 0.25 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC)
  • T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC
  • PD-1 can bind two different but related ligands, PDL-1 and PDL-2
  • Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of &quotedanger signals&quote such as LPS or other molecules associated with bacteria or other pathogens
  • Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases
  • Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.

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Antigen:
CD279 (PD-1)

Concentration:
0.5 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Gene Accession No.:
Q02242

Gene Symbols:
Pdcd1

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
Pdcd1

Clone:
J43

Applications:
Flow Cytometry

Conjugate:
Biotin

Host Species:
Armenian Hamster

Target Species:
Mouse

Gene ID (Entrez):
18566

Isotype:
IgG

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Concentration:
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Monoclonal

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Regulatory Status:
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Gene Accession No.:
P11609

Gene Symbols:
Cd1d1

Primary or Secondary:
Primary

Content And Storage:
4° C

Gene:
Cd1d1

Clone:
1B1

Applications:
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation

Conjugate:
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Primary or Secondary:
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Clone:
145-2C11

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Conjugate:
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Host Species:
Armenian Hamster

Target Species:
Mouse

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12500, 12501, 12502, 12503

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Primary or Secondary:
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Clone:
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