CD3e Monoclonal Antibody (eBio500A2 (500A2)), eBioscience™, Invitrogen™

Description

• Description: The eBio500A2 monoclonal antibody reacts with the 25 kD epsilon subunit of the mouse CD3 complex
• CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex
• CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells, as well as NKT cells
• Binding of eBio500A2 to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation
• The 500A2 antibody is able to partially cross-block the 17A2 and 145-2C11 antibodies, indicating that all three of these anti-CD3 antibodies recognize distinct but overlapping epitopes
• Applications Reported: The eBio500A2 antibody has been reported for use in flow cytometric analysis and immunohistochemistry of frozen tissue
• It has also been reported for use in in vitro activation of mouse T cells
• (Please use Functional Grade purified eBio500A2 in functional assays)
• Applications Tested: This eBio500A2 (500A2) antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex
• T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta
• These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11
• The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits
• CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation
• The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum
• It is never expressed on B or NK cells
• Decreased percentages of T lymphocytes may be observed in some autoimmune diseases
• The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11
• Defects in the CD3 gene are associated with CD3 immunodeficiency.