CD54 (ICAM-1) Monoclonal Antibody (YN1/1.7.4), eBioscience™, Invitrogen™

Description

• Description: The YN1/1.7.4 monoclonal antibody reacts with mouse CD54 (InterCellular Adhesion Molecule-1, ICAM-1), a 90-110 kDa transmembrane glycoprotein expressed by monocytes, lymphocytes, dendritic cells, and endothelial cells
• Expression of CD54 is upregulated on activated lymphocytes
• Interaction of CD54 with its ligands CD11a and CD11b is important in the inflammatory response
• The YN1/1.7.4 monoclonal antibody recognizes a different epitope than the eBioKAT-1 monclonal antibody
• Applications Reported: The YN1/1.7.4 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining
• YN1/1.7.4 has also been reported in blocking of CD54 in functional studies
• (Please use Functional Grade purified YN1/1.7.4, cat
• 16-0541, in functional assays.)
• Applications Tested: The YN1/1.7.4 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain
• ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102)
• ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor
• ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation
• ICAM-1 is also detected in some carcinoma and melanoma cells
• Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.