CD90.1 (Thy-1.1) Monoclonal Antibody (HIS51), eBioscience™, Invitrogen™

Description

• Description: The HIS51 monoclonal antibody reacts with rat CD90 and cross-reacts with mouse CD90.1, a GPI-linked membrane molecule
• In the rat, CD90 is expressed by hematopoietic stem cells, immature B cells, thymocytes, recent thymic emigrants, neurons, inflammed endothelia and other cell types
• In the CD90.1-expressing mouse strains, PL and AKR, CD90 is expressed by early hematopoietic cells in the bone marrow, thymocytes and mature T cells
• Applications Reported: The HIS51 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections
• Applications Tested: The HIS51 antibody has been tested by flow cytometric analysis of rat thymocyte and splenocyte suspensions
• This can be used at less than or equal to 0.125 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family
• It is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts
• Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.