CD140a (PDGFRA) Monoclonal Antibody (APA5), eBioscience™, Invitrogen™

Description

• Description: The APA5 monoclonal antibody reacts with the mouse CD140a molecule, the alpha chain of the platelet derived growth factor receptor (PDGF receptor)
• PDGFRa is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates
• Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity
• CD140a is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues
• Applications Reported: The APA5 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistochemical staining of frozen tissue sections
• It has also been reported in blocking of ligand binding
• (Please use Functional Grade purified APA5 in functional assays.)
• Applications Tested: This APA5 antibody has been tested by flow cytometric analysis of the NIH/3T3 cell line
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• PDGFRA is a cell surface receptor tyrosine kinase which binds members of the platelet-derived growth factor family
• These growth factors are mitogens for cells of mesenchymal origin
• The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides
• Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.