CD144 (VE-cadherin) Monoclonal Antibody (eBioBV14 (BV14)), eBioscience™, Invitrogen™

Description

• Description: The BV14 monoclonal antibody reacts with mouse VE-Cadherin (CD144)
• VE-Cadherin is a 120 kDa member of the type II Cadherin family, characterized by the presence of 5 extracellular cadherin domains (ECD), and anchored to the actin cytoskeleton through their cytoplasmic tail
• VE-Cadherin mediates homophilic adhesion between neighbouring endothelial cells and is localized within specialized structures at cell-cell contacts, called adherens junctions
• VE-Cadherin is expressed constitutively throughout the entire vasculature, and is required for numerous endothelial cell functions including migration, survival, contact-dependent growth inhibition and endothelial cell assembly into tubular structures
• Furthermore, it is thought that VE-Cadherin+CD45- cells from the yolk sac or aorta-gonad-mesonephros (AGM)+ have the potential to give rise to hematopoietic cells
• Cross-blocking experiments suggest that BV14 recognizes a different epitope than another mouse VE-Cadherin monoclonal antibody, BV13
• Applications Reported: This eBioBV14 (BV14) antibody has been reported for use in immunoprecipitation, immunoblotting (WB), and immunohistology staining of frozen tissue sections
• Intravenous injection of BV14 has been shown to induce a concentration- and time-dependent increase in vascular permeability in the heart and lungs, however BV13 was show to be more potent in this activity
• (Please use Functional Grade purified eBioBV13 (BV13), cat
• 16-1441, in functional assays.)
• VE-cadherin is a member of the cadherin superfamily that is located in a six-cadherin cluster in a region on the long arm of chromosome 16 and is involved in loss of heterozygosity events in breast and prostate cancer
• VE-cadherin protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail
• Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, VE-cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions
• An alternative splice variant has been described but the full length sequence of VE-cadherin has not been determined.