7069506
VEGF Receptor 3 Monoclonal Antibody (AFL4), eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
VEGF Receptor 3
Concentration
0.5 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
AI323512; Chy; chylous ascites; Flt4; FLT-4; FLT41; fms related tyrosine kinase 4; FMS-like tyrosine kinase 4; fms-related tyrosine kinase 4; LMPH1A; PCL; receptor protein tyrosine kinase; soluble VEGFR 3; sVEGFR 3; tyrosine-protein kinase receptor FLT4; Vascular endothelial growth factor receptor 3; vascular endothelial growth factor receptor-3; VEGF R3; VEGFR3; VEGFR-3
Gene Symbols
FLT4
Primary or Secondary
Primary
Content And Storage
4° C
Gene
FLT4
Clone
AFL4
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen), Immunoprecipitation, Neutralization, Western Blot
Conjugate
Unconjugated
Host Species
Rat
Target Species
Mouse
Gene Accession No.
P35917
Gene ID (Entrez)
14257
Isotype
IgG2a κ
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: The AFL4 monoclonal antibody reacts with the mouse VEGF receptor-3, also known as Flt-4
- This 195 kDa molecule was identified as an endothelial-specific member of the receptor tyrosine kinase (RTK) family
- During early embryogenesis all endothelial cells express VEGFR-3, while in the adult tissues, VEGFR-3 expression disappears from the vascular endothelial cells and is observed only on the lymphatic endothelium
- However, VEGFR-3 expression is induced in the adult tissue upon tumor implementation suggesting an important role for this receptor in the tumor angiogenesis
- VEGF-C and VEGF-D bind to and activate VEGFR-3
- AFL4 is an antagonist mAb
- Applications Reported: The AFL4 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining of frozen tissue sections
- It has also been reported in blocking of ligand binding
- (Please use Functional Grade purified AFL4, cat
- 16-5988, in functional assays.)
- Applications Tested: The AFL4 antibody has been tested by flow cytometric analysis of in vitro differentiated mouse endothelial cells
- In brief, mouse ES cells were incubated on collagen IV matrix for 4 days and subsequently stimulated with VEGF under serum free conditions to induce further differentiation
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Purity: Greater than 90%, as determined by SDS-PAGE
- Vascular endothelial growth factor is a key regulator of blood vessel development in embryos and angiogenesis in adult tissues
- Unlike VEGF, the related VEGFC stimulates the growth of lymphatic vessels through its specificl ymphatic endothelial receptor VEGFR-3
- In 1998, Dumont showed that targeted inactivation of the VEGFR-3 gene in mice resulted in defective blood vessel development in early embryos
- Vasculogenesis and angiogenesis occurred, but large vessels became abnormally organized with defective lumens, leading to fluid accumulation in the pericardial cavity and cardiovascular failure at embryonic day 9.5
- Thus, VEGFR3 has an essential role in the development of theembryonic cardiovascular system before the emergence of the lymphatic vessels.
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Antigen: VEGF Receptor 3
Concentration: 0.5 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: AI323512; Chy; chylous ascites; Flt4; FLT-4; FLT41; fms related tyrosine kinase 4; FMS-like tyrosine kinase 4; fms-related tyrosine kinase 4; LMPH1A; PCL; receptor protein tyrosine kinase; soluble VEGFR 3; sVEGFR 3; tyrosine-protein kinase receptor FLT4; Vascular endothelial growth factor receptor 3; vascular endothelial growth factor receptor-3; VEGF R3; VEGFR3; VEGFR-3
Gene Symbols: FLT4
Primary or Secondary: Primary
Content And Storage: 4° C
Gene: FLT4
Clone: AFL4
Applications: Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen), Immunoprecipitation, Neutralization, Western Blot
Conjugate: Unconjugated
Host Species: Rat
Target Species: Mouse
Gene Accession No.: P35917
Gene ID (Entrez): 14257
Isotype: IgG2a κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
VEGF Receptor 3
Concentration:
0.5 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
AI323512; Chy; chylous ascites; Flt4; FLT-4; FLT41; fms related tyrosine kinase 4; FMS-like tyrosine kinase 4; fms-related tyrosine kinase 4; LMPH1A; PCL; receptor protein tyrosine kinase; soluble VEGFR 3; sVEGFR 3; tyrosine-protein kinase receptor FLT4; Vascular endothelial growth factor receptor 3; vascular endothelial growth factor receptor-3; VEGF R3; VEGFR3; VEGFR-3
Gene Symbols:
FLT4
Primary or Secondary:
Primary
Content And Storage:
4° C
Gene:
FLT4
Clone:
AFL4
Applications:
Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen), Immunoprecipitation, Neutralization, Western Blot
Conjugate:
Unconjugated
Host Species:
Rat
Target Species:
Mouse
Gene Accession No.:
P35917
Gene ID (Entrez):
14257
Isotype:
IgG2a κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: MHC Class I (H-2Kd/H-2Dd)
Concentration: 0.5 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: class I major histocompatibility antigen H-2Kd; H-2 class I histocompatibility antigen, D-B alpha chain; H-2 class I histocompatibility antigen, K-B alpha chain; H-2 class I histocompatibility antigen, K-D alpha chain; H-2 class I histocompatibility antigen, K-K alpha chain; H-2 class I histocompatibility antigen, K-Q alpha chain; H-2 class I histocompatibility antigen, K-W28 alpha chain; H-2D; H2-D; H-2D cell surface glycoprotein; H-2D(B); H2-D1; H2-DC1-beta transplantation antigen protein; H2DD; H-2K; H2-K; H-2K(B); H-2K(d); H-2K(K); H-2K(Q); H2K1; H2-K1; H2KD; histocompatibility 2, D region locus 1; histocompatibility 2, K1, K region; K-f; MHC class I antigen; MHC class I H2 antigen; MHC class I H-2Dp; MHC class I H2-K-b-alpha-2 cell surface glycoprotein; MHC class I heavy chain H2-K; MHC H2-D-q alpha-chain; MHC H2-K transplantation antigen; Q5k protein
Gene Symbols: H2-D1, H2-K1
Primary or Secondary: Primary
Content And Storage: 4° C
Gene: __
Clone: 34-1-2S
Applications: Flow Cytometry
Conjugate: Unconjugated
Host Species: Mouse
Target Species: Mouse
Gene Accession No.: P01895, P01896, P01897, P01899, P01900, P01901, P03991, P04223, P14426, P14427, P14428
Gene ID (Entrez): 14964, 14972
Isotype: IgG2a κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
MHC Class I (H-2Kd/H-2Dd)
Concentration:
0.5 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
class I major histocompatibility antigen H-2Kd; H-2 class I histocompatibility antigen, D-B alpha chain; H-2 class I histocompatibility antigen, K-B alpha chain; H-2 class I histocompatibility antigen, K-D alpha chain; H-2 class I histocompatibility antigen, K-K alpha chain; H-2 class I histocompatibility antigen, K-Q alpha chain; H-2 class I histocompatibility antigen, K-W28 alpha chain; H-2D; H2-D; H-2D cell surface glycoprotein; H-2D(B); H2-D1; H2-DC1-beta transplantation antigen protein; H2DD; H-2K; H2-K; H-2K(B); H-2K(d); H-2K(K); H-2K(Q); H2K1; H2-K1; H2KD; histocompatibility 2, D region locus 1; histocompatibility 2, K1, K region; K-f; MHC class I antigen; MHC class I H2 antigen; MHC class I H-2Dp; MHC class I H2-K-b-alpha-2 cell surface glycoprotein; MHC class I heavy chain H2-K; MHC H2-D-q alpha-chain; MHC H2-K transplantation antigen; Q5k protein
Gene Symbols:
H2-D1, H2-K1
Primary or Secondary:
Primary
Content And Storage:
4° C
Gene:
__
Clone:
34-1-2S
Applications:
Flow Cytometry
Conjugate:
Unconjugated
Host Species:
Mouse
Target Species:
Mouse
Gene Accession No.:
P01895, P01896, P01897, P01899, P01900, P01901, P03991, P04223, P14426, P14427, P14428
Gene ID (Entrez):
14964, 14972
Isotype:
IgG2a κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: MHC Class I (H-2Db)
Concentration: 0.5 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: H-2 class I histocompatibility antigen, D-B alpha chain; H-2D; H2-D; H-2D cell surface glycoprotein; H-2D(B); H2D1; H2-D1; H2DB; H2-DC1-beta transplantation antigen protein; H2L; H2LD; histocompatibility 2, D region locus 1; MHC class I antigen; MHC class I H2 antigen; MHC class I H-2Dp; MHC H2-D-q alpha-chain; Q5k protein
Gene Symbols: H2-D1
Primary or Secondary: Primary
Content And Storage: 4° C
Gene: H2-D1
Clone: 28-14-8
Applications: Flow Cytometry
Conjugate: Unconjugated
Host Species: Mouse
Target Species: Mouse
Gene Accession No.: P01895, P01896, P01897, P01899, P01900, P14426, P14427
Gene ID (Entrez): 14964
Isotype: IgG2a κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
MHC Class I (H-2Db)
Concentration:
0.5 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
H-2 class I histocompatibility antigen, D-B alpha chain; H-2D; H2-D; H-2D cell surface glycoprotein; H-2D(B); H2D1; H2-D1; H2DB; H2-DC1-beta transplantation antigen protein; H2L; H2LD; histocompatibility 2, D region locus 1; MHC class I antigen; MHC class I H2 antigen; MHC class I H-2Dp; MHC H2-D-q alpha-chain; Q5k protein
Gene Symbols:
H2-D1
Primary or Secondary:
Primary
Content And Storage:
4° C
Gene:
H2-D1
Clone:
28-14-8
Applications:
Flow Cytometry
Conjugate:
Unconjugated
Host Species:
Mouse
Target Species:
Mouse
Gene Accession No.:
P01895, P01896, P01897, P01899, P01900, P14426, P14427
Gene ID (Entrez):
14964
Isotype:
IgG2a κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: Ub-K63
Concentration: 0.5 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: 0610006J14Rik; 2700054O04Rik; 40S ribosomal protein S27a; 60S ribosomal protein L40; AI194771; AL033289; CEP52; CEP80; D8Ertd21e; epididymis luminal protein 112; epididymis secretory protein Li 50; Gm1863; HEL112; HEL-S-50; HMG20; HUBCEP52; L40; Large ribosomal subunit protein eL40; LOC100912032; polyubiquitin B; polyubiquitin C; polyubiquitin-B; Polyubiquitin-C; ribosomal protein S27A; RPL40; Rps27a; S27A; Small ribosomal subunit protein eS31; TI-225; Uba52; UBA80; Ubb; Ubb2; UBC; UBCEP1; UBCEP2; UBCEP80; Ubiquitin; Ubiquitin A-52 residue ribosomal protein fusion product 1; ubiquitin and ribosomal protein S27a; ubiquitin B; ubiquitin C; ubiquitin carboxyl extension protein 52; ubiquitin carboxyl extension protein 80; ubiquitin/60S ribosomal fusion protein; Ubiquitin-40S ribosomal protein S27a; ubiquitin-40S ribosomal protein S27a-like; ubiquitin-52 amino acid fusion protein; ubiquitin-60S ribosomal protein L40; ubiquitin-CEP52; ubiquitin-CEP80; Ubiquitin-related; Ubiquitin-related 1; Ubiquitin-related 2
Gene Symbols: RPS27A, UBA52, UBB, UBC
Primary or Secondary: Primary
Content And Storage: 4° C
Gene: __
Clone: HWA4C4
Applications: Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Target Species: Human
Gene Accession No.: P0CG47, P0CG48, P62979, P62987
Gene ID (Entrez): 6233, 7311, 7314, 7316
Isotype: IgG2a
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
Ub-K63
Concentration:
0.5 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
0610006J14Rik; 2700054O04Rik; 40S ribosomal protein S27a; 60S ribosomal protein L40; AI194771; AL033289; CEP52; CEP80; D8Ertd21e; epididymis luminal protein 112; epididymis secretory protein Li 50; Gm1863; HEL112; HEL-S-50; HMG20; HUBCEP52; L40; Large ribosomal subunit protein eL40; LOC100912032; polyubiquitin B; polyubiquitin C; polyubiquitin-B; Polyubiquitin-C; ribosomal protein S27A; RPL40; Rps27a; S27A; Small ribosomal subunit protein eS31; TI-225; Uba52; UBA80; Ubb; Ubb2; UBC; UBCEP1; UBCEP2; UBCEP80; Ubiquitin; Ubiquitin A-52 residue ribosomal protein fusion product 1; ubiquitin and ribosomal protein S27a; ubiquitin B; ubiquitin C; ubiquitin carboxyl extension protein 52; ubiquitin carboxyl extension protein 80; ubiquitin/60S ribosomal fusion protein; Ubiquitin-40S ribosomal protein S27a; ubiquitin-40S ribosomal protein S27a-like; ubiquitin-52 amino acid fusion protein; ubiquitin-60S ribosomal protein L40; ubiquitin-CEP52; ubiquitin-CEP80; Ubiquitin-related; Ubiquitin-related 1; Ubiquitin-related 2
Gene Symbols:
RPS27A, UBA52, UBB, UBC
Primary or Secondary:
Primary
Content And Storage:
4° C
Gene:
__
Clone:
HWA4C4
Applications:
Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Target Species:
Human
Gene Accession No.:
P0CG47, P0CG48, P62979, P62987
Gene ID (Entrez):
6233, 7311, 7314, 7316
Isotype:
IgG2a
Purification Method:
Affinity chromatography
Product Type:
Antibody