MERTK Monoclonal Antibody (DS5MMER), eBioscience™, Invitrogen™

Description

• Description: This DS5MMER monoclonal antibody recognizes mouse MerTK, a 170-210 kDa member of the TAM family of tyrosine kinase receptors that also includes Axl and Tyro3
• MerTK is expressed on tissue macrophages and is involved in the removal of apoptotic cells
• This process relies on two soluble ligands of MerTK, Protein S and Gas6 that bind to phosphatidylserine found on the outer leaflet of the plasma membrane of cells undergoing apoptosis
• Upon binding these ligands, MerTK undergoes autophosphorylation at multiple tyrosine residues that activate the PI3K and Akt pathways
• This results in the phagocytosis of apoptotic cells and also results in the direct inhibition of TLR-induced production of pro-inflammatory cytokines
• In addition, MerTK may function as a putative entry receptor for filoviruses
• Deficiency of MerTK causes general autoimmunity, inflammation and accumulation of apoptotic bodies
• MerTK is constitutively released from the cell surface by metalloproteinases and thus is present in the serum and culture medium
• This process can be enhanced by stimulation with LPS
• MerTK can be used to help discriminate macrophages from dendritic cells
• MerTK is often expressed on maligt cells and may be implicated in immune evasion
• The DS5MMER antibody will work on paraformaldehyde-fixed cells
• In addition, this antibody was demonstrated to inhibit Gas6-induced MerTK phosphorylation with the IC50 < 5 nM
• MERTK (cMER) is a tyrosine kinase proto-oncogene and is involved in the retinal pigment epithelium (RPE) phagocytosis pathway, which is implicated in human retinal disease
• Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis)
• Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules
• Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1
• MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment
• Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis
• Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.