Aiolos Monoclonal Antibody (8B2), eBioscience™, Invitrogen™

Description

• Description: This 8B2 monoclonal antibody reacts with mouse Aiolos, a 58-kDa member of the Ikaros family of zinc finger transcription factors
• Expression of Aiolos is restricted to the lymphoid lineage, with B cells exhibiting the highest levels of expression, followed by NK and T cells
• Studies demonstrate that Aiolos forms homodimers or heterodimerizes with Ikaros proteins
• Moreover, Aiolos exhibits tyrosine phosphorylation and nuclear localization in response to IL-2 stimulation in T cells
• Aiolos plays a role in IL-2-mediated anti-apoptosis by controlling Bcl-2 expression
• Finally, Aiolos is involved in B cell development, with upregulation of this transcription factor observed at the pre-BI to pre-BII transition in response to pre-B cell receptor signaling
• Applications Reported: This 8B2 antibody has been reported for use in immunoblotting/western blotting
• Applications Tested: This 8B2 antibody has been tested by immunoblotting of reduced cell lysate prepared from mouse splenocytes
• This can be used at less than or equal to 5 μg/mL
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• This gene encodes a member of the Ikaros family of zinc-finger proteins
• Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development
• This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation
• Both Ikaros and Aiolos can participate in chromatin remodeling
• Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers
• At least six alternative transcripts encoding different isoforms have been described.