7069448

Myogenin Monoclonal Antibody (F5D), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

Myogenin

Concentration

0.5 mg/mL

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Alias

bHLHc3; Class C basic helix-loop-helix protein 3; MYF4; Myf-4; myo; MYOD1-related protein; MYOG; Myogenic factor 4; Myogenic factor-4; myogenin; myogenin (myogenic factor 4)

Gene Symbols

MYOG

Primary or Secondary

Primary

Content And Storage

4° C

Gene

MYOG

Clone

F5D

Applications

Immunocytochemistry, Western Blot

Conjugate

Unconjugated

Host Species

Mouse

Target Species

Canine, Human, Mouse, Rat

Gene Accession No.

P12979, P15173, P20428

Gene ID (Entrez)

17928, 29148, 4656, 490224

Isotype

IgG1

Purification Method

Affinity chromatography

Product Type

Antibody

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Description

  • Description: This F5D monoclonal antibody reacts with human, mouse, dog, and rat myogenin, a 34-kDa transcription factor
  • Expressed in skeletal and heart muscle, myogenin is a member of the MyoD family of basic-helix-loop-helix proteins, which also includes MyoD, Myf5, and MRF4
  • This transcription factor interacts with other helix-loop-helix proteins, which may or may not be muscle-specific
  • Myogenin plays a significant role in myogenic differentiation, even directing nonmuscle cells to the myogenic lineage
  • Transforming growth factor-beta (TGFb) and bone morphogenetic protein-2 (BMP2) inhibit myogenin transcriptional activity
  • Predomitly residing within the nucleus, the subcellular localization of myogenin has been shown to be dependent on differentiation status and cell density
  • For instance, trafficking of myogenin between the nucleus and cytoplasm has been reported during skeletal muscle differentiation to mediate transcription control
  • Applications Reported: This F5D antibody has been reported for use in immunoblotting (WB) and immunocytochemistry
  • Applications Tested: This F5D antibody has been tested by western blot and immunocytochemistry of the C2C12 cell line
  • This can be used at less than or equal to 1-5 μg/mL for both applications
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • Purity: Greater than 90%, as determined by SDS-PAGE
  • Aggregation: Less than 10%, as determined by HPLC
  • Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture
  • It is a member of a large family of proteins related by sequence homology, the helix-loop-helix proteins
  • It is essential for the development of functional skeletal muscle.

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7069448

Mouse Monoclonal Antibody...


Antigen:
Myogenin

Concentration:
0.5 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
bHLHc3; Class C basic helix-loop-helix protein 3; MYF4; Myf-4; myo; MYOD1-related protein; MYOG; Myogenic factor 4; Myogenic factor-4; myogenin; myogenin (myogenic factor 4)

Gene Symbols:
MYOG

Primary or Secondary:
Primary

Content And Storage:
4° C

Gene:
MYOG

Clone:
F5D

Applications:
Immunocytochemistry, Western Blot

Conjugate:
Unconjugated

Host Species:
Mouse

Target Species:
Canine, Human, Mouse, Rat

Gene Accession No.:
P12979, P15173, P20428

Gene ID (Entrez):
17928, 29148, 4656, 490224

Isotype:
IgG1

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Armenian Hamster Monoclonal Antibod...


Antigen:
Plexin-B2

Concentration:
0.5 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
Debt; differentially expressed in brain tumors; dJ402G11.3; KIAA0315; MM1; MM1KIAA0315dJ402G11.3; Nbla00445; PLEXB2; plexin B2; plexin-B2; PLXNB2

Gene Symbols:
PLXNB2

Primary or Secondary:
Primary

Content And Storage:
4° C

Gene:
PLXNB2

Clone:
eBio3E7 (3E7)

Applications:
Flow Cytometry, Immunohistochemistry, Immunoprecipitation

Conjugate:
Unconjugated

Host Species:
Armenian Hamster

Target Species:
Mouse

Gene Accession No.:
B2RXS4

Gene ID (Entrez):
140570

Isotype:
IgG

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Rat Monoclonal Antibody...


Antigen:
Ki-67

Concentration:
0.5 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
antigen identified by monoclonal antibody Ki 67; antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67; Antigen KI-67 homolog; antigen KI-67; proliferation marker protein Ki-67; antigen KI-67-like; cb31; D630048A14Rik; I79_022666; Ki67; Ki-67; KIA; LOW QUALITY PROTEIN: proliferation marker protein Ki-67; marker of proliferation Ki-67; MIB-; MIB-1; Mki67; PPP1R105; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2; sb:cb31; si:ch211-250b22.7; unnamed protein product; wu:fa11g09; wu:fb57a07; wu:fi14e05

Gene Symbols:
Mki67

Primary or Secondary:
Primary

Content And Storage:
4° C

Gene:
Mki67

Clone:
SolA15

Applications:
Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Host Species:
Rat

Target Species:
Canine, Cynomolgus Monkey, Human, Mouse, Non-human Primate, Rat

Gene Accession No.:
E9PVX6, P46013

Gene ID (Entrez):
100686578, 102135895, 17345, 291234, 4288

Isotype:
IgG2a κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Antigen:
Ki-67

Concentration:
0.5 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
antigen identified by monoclonal antibody Ki 67; antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67; Antigen KI-67 homolog; antigen KI-67; proliferation marker protein Ki-67; antigen KI-67-like; cb31; D630048A14Rik; I79_022666; Ki67; Ki-67; KIA; LOW QUALITY PROTEIN: proliferation marker protein Ki-67; marker of proliferation Ki-67; MIB-; MIB-1; Mki67; PPP1R105; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2; sb:cb31; si:ch211-250b22.7; unnamed protein product; wu:fa11g09; wu:fb57a07; wu:fi14e05

Gene Symbols:
Mki67

Primary or Secondary:
Primary

Content And Storage:
4° C

Gene:
Mki67

Clone:
20Raj1

Applications:
Immunocytochemistry, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Host Species:
Mouse

Target Species:
Canine, Human

Gene Accession No.:
P46013

Gene ID (Entrez):
100686578, 4288

Isotype:
IgG1 κ

Purification Method:
Affinity chromatography

Product Type:
Antibody