7069471
Nestin Monoclonal Antibody (Rat-401 (Rat401 (4D4))), eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
Nestin
Concentration
0.5 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
AA166324; C78523; ESTM46; FLJ21841; intermediate filament protein; Nbla00170; Nes; Nestin
Gene Symbols
Nes
Primary or Secondary
Primary
Content And Storage
4° C
Gene
Nes
Clone
Rat-401 (Rat401 (4D4))
Applications
Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
Conjugate
Unconjugated
Host Species
Mouse
Target Species
Mouse, Rat
Gene Accession No.
P21263, Q6P5H2
Gene ID (Entrez)
18008, 25491
Isotype
IgG1
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: The monoclonal antibody Rat-401 recognizes mouse and rat nestin, a Class VI intermediate filament protein (>170 kDa) primarily expressed in neuroepithelial stem cells
- Upon neural differentiation, expression is down-regulated and within the adult nervous system is limited to progenitor cells in the cortical subventricular zone, the hippocampal dentate gyrus, dorsal root ganglia satellite cells, and a subpopulation of Schwann cells
- Nestin expression is also present in other progenitor cell populations such as pancreatic islet, angiogenic endothelial cells, and bone marrow mesenchymal stem cells
- Nestin filaments require vimentin and desmin for assembly into heterodimers and polymers
- Upon differentiation, Nestin-containing filaments are replaced by cell type specific intermediate filament proteins, such as GFAP
- Applications Reported: This Rat-401 (Rat401 (4D4)) antibody has been reported for use in western blotting, immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry
- Applications Tested: This Rat-401 antibody has been tested by immunofluorescent staining of paraformaldehyde fixed and permeabilized cells and by immunohistochemistry of formalin-fixed paraffin embedded mouse tissue using low or high pH antigen retrieval
- This can be used at less than or equal to 10 μg/mL
- Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells
- Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells
- Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors
- Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells
- Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.
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Antigen: Nestin
Concentration: 0.5 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: AA166324; C78523; ESTM46; FLJ21841; intermediate filament protein; Nbla00170; Nes; Nestin
Gene Symbols: Nes
Primary or Secondary: Primary
Content And Storage: 4° C
Gene: Nes
Clone: Rat-401 (Rat401 (4D4))
Applications: Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Target Species: Mouse, Rat
Gene Accession No.: P21263, Q6P5H2
Gene ID (Entrez): 18008, 25491
Isotype: IgG1
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
Nestin
Concentration:
0.5 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
AA166324; C78523; ESTM46; FLJ21841; intermediate filament protein; Nbla00170; Nes; Nestin
Gene Symbols:
Nes
Primary or Secondary:
Primary
Content And Storage:
4° C
Gene:
Nes
Clone:
Rat-401 (Rat401 (4D4))
Applications:
Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Target Species:
Mouse, Rat
Gene Accession No.:
P21263, Q6P5H2
Gene ID (Entrez):
18008, 25491
Isotype:
IgG1
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: Nod2
Concentration: 0.5 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: ACUG; BLAU; CARD15; caspase recruitment domain family, member 15; caspase recruitment domain protein 15; caspase recruitment domain-containing protein 15; CD; CLR16.3; F830032C23Rik; IBD1; Inflammatory bowel disease protein 1; NLR family, CARD domain containing 2; Nlrc2; NOD 2; NOD2; NOD-2; NOD2 protein; NOD2B; NOD-like receptor C2; nucleotide binding oligomerization domain containing 2; nucleotide-binding oligomerization domain 2; nucleotide-binding oligomerization domain containing 2; nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 2; nucleotide-binding oligomerization domain-containing protein 2; PSORAS1
Gene Symbols: NOD2
Primary or Secondary: Primary
Content And Storage: 4° C
Gene: NOD2
Clone: 26mNOD2
Applications: Western Blot
Conjugate: Unconjugated
Host Species: Rat
Target Species: Mouse
Gene Accession No.: Q8K3Z0
Gene ID (Entrez): 257632
Isotype: IgG1 κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
Nod2
Concentration:
0.5 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
ACUG; BLAU; CARD15; caspase recruitment domain family, member 15; caspase recruitment domain protein 15; caspase recruitment domain-containing protein 15; CD; CLR16.3; F830032C23Rik; IBD1; Inflammatory bowel disease protein 1; NLR family, CARD domain containing 2; Nlrc2; NOD 2; NOD2; NOD-2; NOD2 protein; NOD2B; NOD-like receptor C2; nucleotide binding oligomerization domain containing 2; nucleotide-binding oligomerization domain 2; nucleotide-binding oligomerization domain containing 2; nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 2; nucleotide-binding oligomerization domain-containing protein 2; PSORAS1
Gene Symbols:
NOD2
Primary or Secondary:
Primary
Content And Storage:
4° C
Gene:
NOD2
Clone:
26mNOD2
Applications:
Western Blot
Conjugate:
Unconjugated
Host Species:
Rat
Target Species:
Mouse
Gene Accession No.:
Q8K3Z0
Gene ID (Entrez):
257632
Isotype:
IgG1 κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: CD366 (TIM3)
Concentration: 0.5 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: CD366; FLJ14428; Havcr2; HAVcr-2; Hepatitis A virus cellular receptor 2; hepatitis A virus cellular receptor 2 homolog; kidney injury molecule-3; KIM-3; sCD366; soluble CD366; soluble TIM 3; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain containing 3; T-cell immunoglobulin and mucin domain-containing protein 3; T-cell immunoglobulin mucin family member 3; T-cell immunoglobulin mucin receptor 3; T-cell membrane protein 3; Tim3; TIM-3; TIMD3; TIMD-3
Gene Symbols: Havcr2
Primary or Secondary: Primary
Content And Storage: 4° C
Gene: Havcr2
Clone: 8B.2C12
Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Conjugate: Unconjugated
Host Species: Rat
Target Species: Mouse
Gene Accession No.: Q8VIM0
Gene ID (Entrez): 171285
Isotype: IgG1 κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
CD366 (TIM3)
Concentration:
0.5 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
CD366; FLJ14428; Havcr2; HAVcr-2; Hepatitis A virus cellular receptor 2; hepatitis A virus cellular receptor 2 homolog; kidney injury molecule-3; KIM-3; sCD366; soluble CD366; soluble TIM 3; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain containing 3; T-cell immunoglobulin and mucin domain-containing protein 3; T-cell immunoglobulin mucin family member 3; T-cell immunoglobulin mucin receptor 3; T-cell membrane protein 3; Tim3; TIM-3; TIMD3; TIMD-3
Gene Symbols:
Havcr2
Primary or Secondary:
Primary
Content And Storage:
4° C
Gene:
Havcr2
Clone:
8B.2C12
Applications:
Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Conjugate:
Unconjugated
Host Species:
Rat
Target Species:
Mouse
Gene Accession No.:
Q8VIM0
Gene ID (Entrez):
171285
Isotype:
IgG1 κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: IgM
Concentration: 0.5 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: __
Gene Alias: __
Gene Symbols: Ighm
Primary or Secondary: Primary
Content And Storage: 4° C
Gene: Ighm
Clone: eB121-15F9
Applications: Flow Cytometry
Conjugate: Unconjugated
Host Species: Rat
Target Species: Mouse
Gene Accession No.: __
Gene ID (Entrez): 16019
Isotype: IgG2a κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
IgM
Concentration:
0.5 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
__
Gene Alias:
__
Gene Symbols:
Ighm
Primary or Secondary:
Primary
Content And Storage:
4° C
Gene:
Ighm
Clone:
eB121-15F9
Applications:
Flow Cytometry
Conjugate:
Unconjugated
Host Species:
Rat
Target Species:
Mouse
Gene Accession No.:
__
Gene ID (Entrez):
16019
Isotype:
IgG2a κ
Purification Method:
Affinity chromatography
Product Type:
Antibody