7069486
GL7 Monoclonal Antibody (GL-7 (GL7)), eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
GL7
Concentration
0.5 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Isotype
IgM
Purification Method
Affinity chromatography
Product Type
Antibody
Clone
GL-7 (GL7)
Applications
Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation
Conjugate
Unconjugated
Host Species
Rat
Target Species
Human, Mouse
Gene Alias
GL7 antigen; Ly77; Ly-77
Primary or Secondary
Primary
Content And Storage
4° C
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Description
- Description: This GL7 monoclonal antibody reacts with a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi
- There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6
- GL7 is commonly used as a marker for mouse germinal center B cells
- The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac
- This moiety is recognized by CD22
- Applications Reported: The GL-7 (GL7) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections
- Applications Tested: The GL-7 (GL7) antibody has been tested by flow cytometric analysis of Con A-stimulated mouse splenocytes
- This can be used at less than or equal to 0.5 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Purity: Greater than 90%, as determined by SDS-PAGE
- Aggregation: Less than 10%, as determined by HPLC
- Filtration: 0.2 μm post-manufacturing filtered
- GL7 is a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi
- There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6
- GL7 is commonly used as a marker for mouse germinal center B cells
- The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac
- This moiety is recognized by CD22.
Compare Similar Items
Show Difference
Antigen: GL7
Concentration: 0.5 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Isotype: IgM
Purification Method: Affinity chromatography
Product Type: Antibody
Clone: GL-7 (GL7)
Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation
Conjugate: Unconjugated
Host Species: Rat
Target Species: Human, Mouse
Gene Alias: GL7 antigen; Ly77; Ly-77
Primary or Secondary: Primary
Content And Storage: 4° C
Antigen:
GL7
Concentration:
0.5 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Isotype:
IgM
Purification Method:
Affinity chromatography
Product Type:
Antibody
Clone:
GL-7 (GL7)
Applications:
Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation
Conjugate:
Unconjugated
Host Species:
Rat
Target Species:
Human, Mouse
Gene Alias:
GL7 antigen; Ly77; Ly-77
Primary or Secondary:
Primary
Content And Storage:
4° C
Antigen: NK1.1
Concentration: 0.5 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Isotype: IgG2a κ
Purification Method: Affinity chromatography
Product Type: Antibody
Clone: PK136
Applications: Flow Cytometry, Immunoprecipitation
Conjugate: Unconjugated
Host Species: Mouse
Target Species: Mouse
Gene Alias: AI462337; CD161; CD161 antigen-like family member B; CD161 antigen-like family member C; CD161b; CD161c; EMBL:AAQ11375.1}; Inhibitory receptor NKR-P1B; killer cell lectin-like receptor subfamily A member 1C; killer cell lectin-like receptor subfamily B member 1B; killer cell lectin-like receptor subfamily B member 1B allele A; Killer cell lectin-like receptor subfamily B member 1B allele B; killer cell lectin-like receptor subfamily B member 1B allele C; killer cell lectin-like receptor subfamily B member 1C; killer cell lectin-like receptor subfamily B member 1D; killer cell lectin-like receptor subfamily B member 1F; KLRB1; Klrb1b; klrb1b {ECO:0000312; Klrb1c; Klrb1d; Klrb1f; klrb1f {ECO:0000250; Ly55; Ly-55; Ly55b; ly-55b; Ly55c; ly-55c; Ly55d; Ly-55d; Ly59; Ly-59; lymphocyte antigen 55 complex, locus B; lymphocyte antigen 55 complex, locus C; lymphocyte antigen 55 complex, locus D; lymphocyte antigen 55b; lymphocyte antigen 55c; Lymphocyte antigen 55d; lymphocyte antigen 59; MGC163757; MGC163759; natural killer cell receptor protein NKR-P1B; natural killer cell receptor protein NKR-P1C; natural killer cell receptor-P1; natural killer cell surface protein NKR-P1B allele B6; Natural killer cell surface protein NKR-P1B allele RNK/SD/BN/F344; natural killer cell surface protein NKR-P1B allele SJL/BALB; natural killer cell surface protein NKR-P1B allele TO; natural killer cell surface protein NKR-P1F; Natural killer cell surface protein P1-40; natural killer cell-associated antigen 1; natural killer cells; nk cells; Nk1; Nk-1; NK1.1; Nk1.2; Nk-1.2; NKRP1; NK-RP1; NKR-P1 34; NKR-P1 40; NKR-P1.9; NKRP140; Nkrp1b; NKR-P1B; Nkrp1-b; NKR-P1B protein; Nkrp1c; NKR-P1C; Nkrp1d; NKR-P1D; Nkrp1f; NKR-P1F; RGD:2975}; UniProtKB:Q8VD98}
Primary or Secondary: Primary
Content And Storage: 4° C
Antigen:
NK1.1
Concentration:
0.5 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Isotype:
IgG2a κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Clone:
PK136
Applications:
Flow Cytometry, Immunoprecipitation
Conjugate:
Unconjugated
Host Species:
Mouse
Target Species:
Mouse
Gene Alias:
AI462337; CD161; CD161 antigen-like family member B; CD161 antigen-like family member C; CD161b; CD161c; EMBL:AAQ11375.1}; Inhibitory receptor NKR-P1B; killer cell lectin-like receptor subfamily A member 1C; killer cell lectin-like receptor subfamily B member 1B; killer cell lectin-like receptor subfamily B member 1B allele A; Killer cell lectin-like receptor subfamily B member 1B allele B; killer cell lectin-like receptor subfamily B member 1B allele C; killer cell lectin-like receptor subfamily B member 1C; killer cell lectin-like receptor subfamily B member 1D; killer cell lectin-like receptor subfamily B member 1F; KLRB1; Klrb1b; klrb1b {ECO:0000312; Klrb1c; Klrb1d; Klrb1f; klrb1f {ECO:0000250; Ly55; Ly-55; Ly55b; ly-55b; Ly55c; ly-55c; Ly55d; Ly-55d; Ly59; Ly-59; lymphocyte antigen 55 complex, locus B; lymphocyte antigen 55 complex, locus C; lymphocyte antigen 55 complex, locus D; lymphocyte antigen 55b; lymphocyte antigen 55c; Lymphocyte antigen 55d; lymphocyte antigen 59; MGC163757; MGC163759; natural killer cell receptor protein NKR-P1B; natural killer cell receptor protein NKR-P1C; natural killer cell receptor-P1; natural killer cell surface protein NKR-P1B allele B6; Natural killer cell surface protein NKR-P1B allele RNK/SD/BN/F344; natural killer cell surface protein NKR-P1B allele SJL/BALB; natural killer cell surface protein NKR-P1B allele TO; natural killer cell surface protein NKR-P1F; Natural killer cell surface protein P1-40; natural killer cell-associated antigen 1; natural killer cells; nk cells; Nk1; Nk-1; NK1.1; Nk1.2; Nk-1.2; NKRP1; NK-RP1; NKR-P1 34; NKR-P1 40; NKR-P1.9; NKRP140; Nkrp1b; NKR-P1B; Nkrp1-b; NKR-P1B protein; Nkrp1c; NKR-P1C; Nkrp1d; NKR-P1D; Nkrp1f; NKR-P1F; RGD:2975}; UniProtKB:Q8VD98}
Primary or Secondary:
Primary
Content And Storage:
4° C
Antigen: CD253 (TRAIL)
Concentration: 0.5 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Isotype: IgG2a κ
Purification Method: Affinity chromatography
Product Type: Antibody
Clone: N2B2
Applications: Flow Cytometry, Functional Assay, Immunoprecipitation, Neutralization
Conjugate: Unconjugated
Host Species: Rat
Target Species: Mouse
Gene Alias: A330042I21Rik; AI448571; Apo-2 ligand; APO2L; Apo-2L; CD253; CD253 antigen; chemokine tumor necrosis factor ligand superfamily member 10; Ly81; Protein TRAIL; TL2; TN; TNF superfamily member 10; TNF-related apoptosis inducing ligand; TNF-related apoptosis inducing ligand TRAIL; TNF-related apoptosis-inducing ligand; Tnfsf10; TNLG6A; TRAIL; TRAIL protein; TRAIL/APO2L; tumor necrosis factor (ligand) family, member 10; tumor necrosis factor (ligand) superfamily member 10; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor apoptosis-inducing ligand splice variant delta; tumor necrosis factor ligand 6A; tumor necrosis factor ligand superfamily member 10; tumor necrosis factor superfamily member 10
Primary or Secondary: Primary
Content And Storage: 4° C
Antigen:
CD253 (TRAIL)
Concentration:
0.5 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Isotype:
IgG2a κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Clone:
N2B2
Applications:
Flow Cytometry, Functional Assay, Immunoprecipitation, Neutralization
Conjugate:
Unconjugated
Host Species:
Rat
Target Species:
Mouse
Gene Alias:
A330042I21Rik; AI448571; Apo-2 ligand; APO2L; Apo-2L; CD253; CD253 antigen; chemokine tumor necrosis factor ligand superfamily member 10; Ly81; Protein TRAIL; TL2; TN; TNF superfamily member 10; TNF-related apoptosis inducing ligand; TNF-related apoptosis inducing ligand TRAIL; TNF-related apoptosis-inducing ligand; Tnfsf10; TNLG6A; TRAIL; TRAIL protein; TRAIL/APO2L; tumor necrosis factor (ligand) family, member 10; tumor necrosis factor (ligand) superfamily member 10; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor apoptosis-inducing ligand splice variant delta; tumor necrosis factor ligand 6A; tumor necrosis factor ligand superfamily member 10; tumor necrosis factor superfamily member 10
Primary or Secondary:
Primary
Content And Storage:
4° C
Antigen: AID
Concentration: 0.5 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Isotype: IgG2a κ
Purification Method: Affinity chromatography
Product Type: Antibody
Clone: mAID-2
Applications: Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
Conjugate: Unconjugated
Host Species: Rat
Target Species: Human, Mouse
Gene Alias: activation induced deaminase; activation-induced cytidine deaminase; Aicda; AID; Arp2; CDA2; Cytidine aminohydrolase; epididymis secretory protein Li 284; HEL-S-284; HIGM2; integrated into Burkitt's lymphoma cell line Ramos; single-stranded DNA cytosine deaminase
Primary or Secondary: Primary
Content And Storage: 4° C
Antigen:
AID
Concentration:
0.5 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Isotype:
IgG2a κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Clone:
mAID-2
Applications:
Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
Conjugate:
Unconjugated
Host Species:
Rat
Target Species:
Human, Mouse
Gene Alias:
activation induced deaminase; activation-induced cytidine deaminase; Aicda; AID; Arp2; CDA2; Cytidine aminohydrolase; epididymis secretory protein Li 284; HEL-S-284; HIGM2; integrated into Burkitt's lymphoma cell line Ramos; single-stranded DNA cytosine deaminase
Primary or Secondary:
Primary
Content And Storage:
4° C