MASH1 Monoclonal Antibody (24B72D11), eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody 24B72D11 recognizes mouse and rat Mammalian achaete-scute homolog 1 (MASH1), a 34 kDa basic helix-loop-helix (bHLH) transcription factor
• MASH1, like family members Olig2 and Ngn2, is expressed in progenitor cells throughout the nervous system and retina and is a key regulator of cell fate specification
• Expression of MASH1 is regulated by the Delta/Notch1 pathway through direct binding of Hes1 to MASH1 as well as competitive binding to MASH1 hetero-dimerizing proteins
• At a transcriptional level, MASH1 expression is controlled by notch-mediated regulation of sox2, which binds to the MASH1 promoter region up-regulating expression
• MASH1 has been implicated in the formation of multiple neural cell types including dopaminergic, serotonergic, glutaminergic and GABAergic neurons
• Overexpression of MASH1 has been shown in small cell lung carcinoma and medullary thyroid cancer
• Applications Reported: This 24B72D11 antibody has been reported for use in western blotting, immunohistochemical staining of frozen tissue sections, and immunocytochemistry
• Applications Tested: This 24B72D11 antibody has been tested by western blot on reduced PC12 cells and can be used at less than or equal to 5 μg/mL
• The 24B72D11 antibody has also been tested by immunocytochemistry on fixed and permeablized PC12 cells at can be used at less than or equal to 20 μg/mL
• ASCL1 (also known as ASH1) is a basic helix-loop-helix transcription factor that is required for early development of the nervous system
• Expressed in fetal brain, ASCL1 is essential for the proper development of autonomic neurons and for the survival of subsets of autonomic neurons
• ASCL1 interaction with MEF-2A may regulate the expression of specific genes that are critical for the formation of distinct neuronal circuits within the central nervous system
• The high level of ASCL1 expression in neuroendocrine tumors, such as medullary thyroid cancer, small cell lung cancer and lung cancer with neuroendocrine features may provide a useful marker for cancers with neuroendocrine features
• Mapping to human chromosome 12, the ASCL1 gene contains a trinucleotide repeat region, making this locus a candidate for inherited disease.