FOXP3 Monoclonal Antibody (150D/E4), eBioscience™, Invitrogen™

Description

• Description: eBioscience offers a panel of monoclonal antibodies to different epitopes of human/primate and mouse/rat Foxp3 protein, providing useful tools for investigating the complete expression pattern of Foxp3 at the protein level, and discerning the precise subsets of Foxp3+ cells
• Other antibodies to Foxp3 available from eBioscience, which have been used significantly in scientific literature, include the anti-human Foxp3 PCH101 (cat
• 72-5776) and ebio7979 (cat
• 12-7979), and the anti-mouse/rat Foxp3 FJK-16s (cat
• 72-5775)
• The 150D/E4 has been mapped to the splice region in Exon 2 found in human cells
• The splicing of this region has not been shown to occur in mouse
• Characterization of this antibody to the splice variant has been limited to epitope mapping
• It should be noted that other antibodies such as PCH101 and 236A/E7 will recognize both the spliced and full length forms of the Foxp3 protein and stain more intensely than 150D/E4 and FJK-16s in human cells
• The 150D/E4 antibody reacts with mouse/rat/human Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2
• Cross reactivity of this antibody to other proteins has not been determined
• Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice
• FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis
• FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells
• FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells
• Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice
• In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome
• Transcript variants of FOXP3 encoding different isoforms have been identified
• In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.