IL-2 Monoclonal Antibody (JES6-1A12), eBioscience™, Invitrogen™

Description

• Description: The JES6-1A12 antibody reacts with mouse interleukin-2 (IL-2)
• The JES6-1A12 antibody is a neutralizing antibody
• Mouse IL-2 (also known as TCGF) is a 17 kDa factor produced mainly by activated CD4+ T cells
• IL-2 induces cell cycle progression of resting cells in an antigen non-specific manner and allows clonal expansion of activated T cells
• IL-2 also acts on activated B cells, monocytes, NK cells, LAK cells, and on oligodendroglial cells in vitro
• In addition, IL-2 plays a role in hematopoiesis, tumor surveillance and anti-inflammatory reactions, and hence is a central regulator of the immune response
• Non-glycosylated IL-2 is biologically active
• Applications Reported: The JES6-1A12 antibody has been reported for use in ELISA capture, ELISPOT capture, neutralization of IL-2 bioactivity, IP, and intracellular staining
• Applications Tested: The JES6-1A12 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse Interleukin-2 (IL-2) in combination with the biotin JES6-5H4 (13-7021) antibody for detection and recombit mouse IL-2 (14-8021) as the standard
• A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL
• A standard curve consisting of doubling dilutions of the recombit standard over the range of 500 pg/mL - 4 pg/mL should be included in each ELISA plate
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages
• IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7)
• The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene
• The targeted disruption of a gene similar to IL-2 in mice leads to an μgcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.